Evidence map›Paper›PMID 36318391›Full record

ArticleJournal, genetic engineering & biotechnology2022

Assessment of genetic diversity among wild rose in Morocco using ISSR and DAMD markers.

Karim Saghir, Rabha Abdelwahd, Driss Iraqi, Nezha Lebkiri, Fatima Gaboun, Younes El Goumi, Maha Ibrahimi, Younes Abbas, Ghizlane Diria

Open access · diamondAbstract read
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Article in Journal, genetic engineering & biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Karim SaghirBiotechnology Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research (INRA), Rabat, Morocco.
Rabha AbdelwahdBiotechnology Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research (INRA), Rabat, Morocco.
Driss IraqiBiotechnology Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research (INRA), Rabat, Morocco.
Nezha LebkiriBiotechnology Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research (INRA), Rabat, Morocco.
Fatima GabounBiotechnology Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research (INRA), Rabat, Morocco.
Younes El GoumiPolyvalent Team in R&D, Higher School of Technology of Fkih Ben-Salah, Sultan Moulay Slimane University, USMS, 23000, Beni Mellal, Morocco.
Maha IbrahimiBiotechnology Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research (INRA), Rabat, Morocco.
Younes AbbasPolyvalent team in R&D, Polydisciplinary Faculty, Sultan Moulay Slimane University, USMS, 23000, Beni Mellal, Morocco. a.younes@usms.ma.
Ghizlane DiriaBiotechnology Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research (INRA), Rabat, Morocco. ghizlane.diria@inra.ma.
Université Sultan Moulay Slimane · MAUniversité Ibn-Tofail · MA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMorocco is considered one of the main biodiversity hotspots in the Mediterranean region and contains various plant species including wild and domestic Rosa. This genus is the most important among cultivated ornamental plants in the world, with a high economic value in cosmetics, pharmaceutical industries, and floriculture. In the present study, genetic diversity among the collected accessions of wild Rosa species in Morocco was assessed using Inter-Simple Sequence Repeat (ISSR) and Directed Amplification of Minisatellites DNA (DAMD) markers.

resultsResults confirmed that both markers used have a good efficiency to assess genetic diversity in wild roses. Ten ISSR and eight DAMD primers amplified 276 and 203 loci, with an average of 27.4 and 25 polymorphic alleles per primer, respectively. The polymorphic information content (PIC) values were 0.34 with ISSR and 0.31 with DAMD. Analysis of molecular variance (AMOVA) showed that genetic variation in wild rose occurs mainly within populations (86%) rather than between populations (14%). The region of Azrou (Middle Atlas of Morocco) is the area that registered the highest genetic diversity in the present study with He = 0.21. The 39 rose accessions were divided into three main groups with among-group similarity of 30%. Principal component analysis and the hierarchical classification were consistent with genetic relationships derived by structure analysis.

conclusionThe findings revealed that the patterns of grouping are weakly correlated with geographical origin. ISSR and DAMD markers showed that the accessions have a good genetic diversity.

Indexed as

DAMDGenetic diversityISSRPopulation structureWild rose

Identifiers

PMID36318391
PMCPMC9626730
OpenAlexW4307971905

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.