Evidence map›Paper›PMID 36317965›Full record

ArticleeLife2022

Molecular and anatomical characterization of parabrachial neurons and their axonal projections.

Jordan L Pauli, Jane Y Chen, Marcus L Basiri, Sekun Park, Matthew E Carter, Elisenda Sanz, G Stanley McKnight, Garret D Stuber, Richard D Palmiter

Open access · goldAbstract read
In one paragraph

Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.

0numbers the graph read from it
0cells of the map it votes in
96citing papers in PubMed
10.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

96 citing papers in PubMed, 134 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
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  8. Article
  9. ParabrachialProceedings of the National Academy of Sciences of the United States of America · 2026
    Article
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  16. Intersectional expression ofAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2026
    Article
  17. A Tac1-Expressing Brainstem Pathway Underlies the Pathogenesis of Trigeminal Neuralgia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  18. Review
  19. Article
  20. Parabrachial CGRP Neurons Regulate Opioid Reinforcement.bioRxiv : the preprint server for biology · 2026
    Article

36 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jordan L Pauli *Department of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United States.ORCID 0000-0001-6276-3407
Jane Y Chen *Department of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United States.ORCID 0000-0002-3986-8785
Marcus L Basiri *Center for the Neurobiology of Addiction, Pain, and Emotion, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, United States.ORCID 0000-0002-4829-7187
Sekun ParkDepartment of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United States.
Matthew E CarterDepartment of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United States.ORCID 0000-0003-1802-090X
Elisenda SanzDepartment of Pharmacology, University of Washington, Seattle, United States.ORCID 0000-0002-7932-8556
G Stanley McKnightDepartment of Pharmacology, University of Washington, Seattle, United States.
Garret D StuberCenter for the Neurobiology of Addiction, Pain, and Emotion, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, United States.ORCID 0000-0003-1730-4855
Richard D PalmiterDepartment of Biochemistry, Howard Hughes Medical Institute, University of Washington, Seattle, United States.ORCID 0000-0001-6587-0582
Howard Hughes Medical Institute · USUniversity of Washington · US

Funding

University of Washington Center of Excellence in Opioid Addiction ResearchP30DA048736 · NIDA · UNIVERSITY OF WASHINGTON · PI Charles Chavkin · 2019 to 2026
$13.0M
Lateral Hypothalamic Circuits for Feeding and ReinforcementR01DA038168 · NIDA · UNIV OF NORTH CAROLINA CHAPEL HILL · PI STUBER, GARRET D · 2014 to 2025
$4.0M
Effect of Killing or Removing GABA from NPY/AgRP NeuronsR01DA024908 · NIDA · UNIVERSITY OF WASHINGTON · PI PALMITER, RICHARD D. · 2007 to 2021
$3.5M
Midbrain Neural Circuit Elements That Underlie Cue-Reward AssociationsR01DA032750 · NIDA · UNIV OF NORTH CAROLINA CHAPEL HILL · PI STUBER, GARRET D · 2012 to 2016
$1.6M
NIDA NIH HHS R01 DA024908NIDA NIH HHS R01 DA032750NIDA NIH HHS R01 DA038168
6 · The paper itself

Abstract

The parabrachial nucleus (PBN) is a major hub that receives sensory information from both internal and external environments. Specific populations of PBN neurons are involved in behaviors including food and water intake, nociceptive responses, breathing regulation, as well as learning and responding appropriately to threatening stimuli. However, it is unclear how many PBN neuron populations exist and how different behaviors may be encoded by unique signaling molecules or receptors. Here we provide a repository of data on the molecular identity, spatial location, and projection patterns of dozens of PBN neuron subclusters. Using single-cell RNA sequencing, we identified 21 subclusters of neurons in the PBN and neighboring regions. Multiplexed in situ hybridization showed many of these subclusters are enriched within specific PBN subregions with scattered cells in several other regions. We also provide detailed visualization of the axonal projections from 21 Cre-driver lines of mice. These results are all publicly available for download and provide a foundation for further interrogation of PBN functions and connections.

Indexed as

Parabrachial NucleusAnimalsAxonsMiceNeuronsmousemultiplex in situ hybridizationneuroscienceparabrachial nucleusscRNA sequencing

Identifiers

PMID36317965
PMCPMC9668336
OpenAlexW4307848524

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.