Evidence map›Paper›PMID 36317879›Full record

ArticleJournal of virology2022

hnRNP K Degrades Viral Nucleocapsid Protein and Induces Type I IFN Production to Inhibit Porcine Epidemic Diarrhea Virus Replication.

Wenzhen Qin, Ning Kong, Chunmei Wang, Sujie Dong, Huanjie Zhai, Xueying Zhai, Xinyu Yang, Chenqian Ye, Manqing Ye, Wu Tong and 8 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 3 institutions in 1 country.

Wenzhen Qin *Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Ning Kong *Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Chunmei Wang *Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Sujie DongShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Huanjie ZhaiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Xueying ZhaiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Xinyu YangShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Chenqian YeShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Manqing YeShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Wu TongShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Changlong LiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Lingxue YuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Hao ZhengShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Hai YuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Daoliang LanCollege of Animal & Verterinary Sciences, Southwest Minzu Universitygrid.412723.1, Chengdu, China.
Wen ZhangSchool of Medicine, Jiangsu Universitygrid.440785.a, Zhenjiang, China.
Guangzhi TongShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Tongling ShanShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.ORCID 0000-0002-5329-6349
Shanghai Veterinary Research Institute · CNJiangsu University · CNSouthwest Minzu University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) is a re-emerging enteric coronavirus currently spreading in several nations and inflicting substantial financial damages on the swine industry. The currently available coronavirus vaccines do not provide adequate protection against the newly emerging viral strains. It is essential to study the relationship between host antiviral factors and the virus and to investigate the mechanisms underlying host immune response against PEDV infection. This study shows that heterogeneous nuclear ribonucleoprotein K (hnRNP K), the host protein determined by the transcription factor KLF15, inhibits the replication of PEDV by degrading the nucleocapsid (N) protein of PEDV in accordance with selective autophagy. hnRNP K was found to be capable of recruiting the E3 ubiquitin ligase, MARCH8, aiming to ubiquitinate N protein. Then, it was found that the ubiquitinated N protein could be delivered into autolysosomes for degradation by the cargo receptor NDP52, thereby inhibiting PEDV proliferation. Moreover, based on the enhanced MyD88 expression, we found that hnRNP K activated the interferon 1 (IFN-1) signaling pathway. Overall, the data obtained revealed a new mechanism of hnRNP K-mediated virus restriction wherein hnRNP K suppressed PEDV replication by degradation of viral N protein using the autophagic degradation pathway and by induction of IFN-1 production based on upregulation of MyD88 expression.

Indexed as

Coronavirus InfectionsHeterogeneous-Nuclear Ribonucleoprotein KInterferon Type IPorcine epidemic diarrhea virusVirus ReplicationAnimalsAntiviral AgentsChlorocebus aethiopsInterferonsMyeloid Differentiation Factor 88Nucleocapsid ProteinsSwineSwine DiseasesUbiquitin-Protein LigasesVero CellsAntiviral AgentsHeterogeneous-Nuclear Ribonucleoprotein KInterferonsInterferon Type IMyeloid Differentiation Factor 88Nucleocapsid ProteinsUbiquitin-Protein LigaseshnRNP KIFN-1MARCH8NDP52N proteinPEDVselective autophagy

Identifiers

PMID36317879
PMCPMC9682996
OpenAlexW4307844777

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.