Evidence map›Paper›PMID 36317866›Full record

ArticleThe journal of physical chemistry. B2022

Unraveling Complex Local Protein Environments with 4-Cyano-l-phenylalanine.

ByungUk Lee, Brianna M Papoutsis, Nathan Y Wong, Juliana Piacentini, Caroline Kearney, Nia A Huggins, Nicole Cruz, Tracey T Ng, Kexin Heather Hao, Jeremy S Kramer and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of physical chemistry. B, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Synthesis and In Vivo Incorporation of 4-Difluoromethyl-L-Phenylalanine Into Proteins.Chembiochem : a European journal of chemical biology · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

ByungUk LeeDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.
Brianna M PapoutsisDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.
Nathan Y WongDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.
Juliana PiacentiniDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.
Caroline KearneyDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.
Nia A HugginsDepartment of Biological Sciences, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032, United States.
Nicole CruzDepartment of Biological Sciences, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032, United States.
Tracey T NgDepartment of Physics and Astronomy, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032, United States.
Kexin Heather HaoDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.
Jeremy S KramerDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.
Edward E FenlonDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.ORCID 0000-0002-6701-8163
Paul S NerenbergDepartment of Biological Sciences, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032, United States.ORCID 0000-0002-9730-6983
Christine M Phillips-PiroDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.ORCID 0000-0002-7335-7487
Scott H BrewerDepartment of Chemistry, Franklin & Marshall College, P.O. Box 3003, Lancaster, Pennsylvania 17604-3003, United States.
Franklin & Marshall College · USCalifornia State University Los Angeles · US

Funding

Overall EvaluationP41GM103403 · NIGMS · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2012 to 2017
$14.3M
Use of Vibrational Reporters to Measure Dynamics and Distances in RNA and ProteinsR15GM093330 · NIGMS · FRANKLIN AND MARSHALL COLLEGE · PI BREWER, SCOTT H, FENLON, EDWARD EMMETT · 2010 to 2024
$1.3M
NIGMS NIH HHS P41 GM103403NIGMS NIH HHS R15 GM093330
6 · The paper itself

Abstract

We present a multifaceted approach to effectively probe complex local protein environments utilizing the vibrational reporter unnatural amino acid (UAA) 4-cyano-l-phenylalanine (pCNPhe) in the model system superfolder green fluorescent protein (sfGFP). This approach combines temperature-dependent infrared (IR) spectroscopy, X-ray crystallography, and molecular dynamics (MD) simulations to provide a molecular interpretation of the local environment of the nitrile group in the protein. Specifically, a two-step enantioselective synthesis was developed that provided an 87% overall yield of pCNPhe in high purity without the need for chromatography. It was then genetically incorporated individually at three unique sites (74, 133, and 149) in sfGFP to probe these local protein environments. The incorporation of the UAA site-specifically in sfGFP utilized an engineered, orthogonal tRNA synthetase in

Indexed as

PhenylalanineAmino AcidsEscherichia coliGreen Fluorescent ProteinsHydrogenNitrilesAmino AcidsGreen Fluorescent ProteinsHydrogenNitrilesp-cyanophenylalaninePhenylalanine

Identifiers

PMID36317866
PMCPMC10234312
OpenAlexW4307839837

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.