Evidence map›Paper›PMID 36317179›Full record

ArticleSTAR protocols2022

Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells.

Ana Luiza Drumond-Bock, Magdalena Cybula, Luyao Wang, Lin Wang, Magdalena Bieniasz

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ana Luiza Drumond-BockOklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. Electronic address: analuiza-bock@omrf.org.
Magdalena CybulaCytovance® Biologics, Oklahoma City, OK 73104, USA.
Luyao WangOklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
Lin WangOklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
Magdalena BieniaszOklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. Electronic address: magdalena-bieniasz@omrf.org.
Oklahoma Medical Research Foundation · US

Funding

Targeting sfRon-S6K1 signaling and mitotic kinesin Eg5 in ovarian cancer: a novel synergistic treatment strategyR21CA264573 · NCI · OKLAHOMA MEDICAL RESEARCH FOUNDATION · PI BIENIASZ, MAGDALENA · 2021 to 2022
$445k
NCI NIH HHS R21 CA264573
6 · The paper itself

Abstract

Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures. The present protocol defines a specific strategy to amplify BRD4-L, followed by the successful cloning of the gene into an overexpression vector. Since there are no existing protocols nor commercially available plasmids, this work provides a useful tool for studies involving molecular cloning of BRD4-L and could potentially be applied to other challenging genes.

Indexed as

Genetic VectorsNuclear ProteinsAnimalsCloning, MolecularMammalsProtein IsoformsTranscription FactorsNuclear ProteinsProtein IsoformsTranscription FactorsCell biologyMolecular biologyProtein biochemistryProtein expression and purification

Identifiers

PMID36317179
PMCPMC9617197
OpenAlexW4307974479

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.