SynthesisNPJ biofilms and microbiomes2022
Exploring species-level infant gut bacterial biodiversity by meta-analysis and formulation of an optimized cultivation medium.
Synthesis in NPJ biofilms and microbiomes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 28 citations in OpenAlex.
- Fecal Microbiota Transplantation from Toddler Donors Ameliorated DSS-Induced Colitis in Mice by Reshaping Gut Microbiota.Nutrients · 2026Article
- A bifidobacterial enzyme orchestrates ecology and function of infant gut bacterial community.bioRxiv : the preprint server for biology · 2026Article
- Early-Life Gut Microbiota: Education of the Immune System and Links to Autoimmune Diseases.Microorganisms · 2026Review
- Compiling an early life human gut microbiome atlas and identification of key microbial drivers.NPJ biofilms and microbiomes · 2025Article
- Quorum Sensing via Autoinducer-2 Promotes Colonisation and Host Adaptation in B. bifidum PRL2010.Microbial biotechnology · 2025Article
- Functional modulation of the human gut microbiome by bacteria vehicled by cheese.Applied and environmental microbiology · 2025Article
- Impact of Ex Vivo Bisphenol A Exposure on Gut Microbiota Dysbiosis and Its Association with Childhood Obesity.Journal of xenobiotics · 2025Article
- Disclosing the effects of pea-derived proteins on the human gut microbiota.Microbiome research reports · 2025Article
- Understanding the interaction between melatonin and bifidobacteria.Microbiome research reports · 2025Article
- Dissecting the molecular interactions between botanical extracts and the human gut microbiota.Frontiers in microbiology · 2025Article
- Characterization of aApplied and environmental microbiology · 2024Article
- Disclosing α-lactalbumin impact on the intestinal and vaginal microbiota of women suffering from polycystic ovary syndrome.Microbial biotechnology · 2024Article
- Impact of cryoprotective agents on human gut microbes and in vitro stabilized artificial gut microbiota communities.Microbial biotechnology · 2024Article
- Species-level characterization of saliva and dental plaque microbiota reveals putative bacterial and functional biomarkers of periodontal diseases in dogs.FEMS microbiology ecology · 2024Article
- Advances in Culturomics Research on the Human Gut Microbiome: Optimizing Medium Composition and Culture Techniques for Enhanced Microbial Discovery.Journal of microbiology and biotechnology · 2024Review
- Taxonomic and metabolic development of the human gut microbiome across life stages: a worldwide metagenomic investigation.mSystems · 2024Article
- GH136-encoding gene (perB) is involved in gut colonization and persistence by Bifidobacterium bifidum PRL2010.Microbial biotechnology · 2024Article
- Molecular cross-talk among human intestinal bifidobacteria as explored by a human gut model.Frontiers in microbiology · 2024Article
- A flexible high-throughput cultivation protocol to assess the response of individuals' gut microbiota to diet-, drug-, and host-related factors.ISME communications · 2024Article
- What Makes the Gut-Lung Axis Working? From the Perspective of Microbiota and Traditional Chinese Medicine.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2024Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro gut cultivation models provide host-uncoupled, fast, and cost-efficient solutions to investigate the effects of intrinsic and extrinsic factors impacting on both composition and functionality of the intestinal microbial ecosystem. However, to ensure the maintenance and survival of gut microbial players and preserve their functions, these systems require close monitoring of several variables, including oxygen concentration, pH, and temperature, as well as the use of a culture medium satisfying the microbial nutritional requirements. In this context, in order to identify the macro- and micro-nutrients necessary for in vitro cultivation of the infant gut microbiota, a meta-analysis based on 1669 publicly available shotgun metagenomic samples corresponding to fecal samples of healthy, full-term infants aged from a few days to three years was performed to define the predominant species characterizing the "infant-like" gut microbial ecosystem. A subsequent comparison of growth performances was made using infant fecal samples that contained the most abundant bacterial taxa of the infant gut microbiota, when cultivated on 18 different culture media. This growth analysis was performed by means of flow cytometry-based bacterial cell enumeration and shallow shotgun sequencing, which allowed the formulation of an optimized growth medium, i.e., Infant Gut Super Medium (IGSM), which maintains and sustains the infant gut microbial biodiversity under in vitro growth conditions. Furthermore, this formulation was used to evaluate the in vitro effect of two drugs commonly used in pediatrics, i.e., acetaminophen and simethicone, on the taxonomic composition of the infant gut microbiota.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.