Evidence map›Paper›PMID 36314793›Full record

ArticleJournal of clinical microbiology2022

Where Have All the Diagnostic Morphological Parasitologists Gone?

Richard S Bradbury, Sarah G H Sapp, Idzi Potters, Blaine A Mathison, John Frean, Abhishek Mewara, Harsha Sheorey, Francesca Tamarozzi, Marc Roger Couturier, Peter Chiodini and 1 more

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
9.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 59 citations in OpenAlex.

  1. Development and validation of the AI-predictive ParaScoutEmerging microbes & infections · 2026
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  5. Entamoeba histolytica: Diagnostic Microscopy in Clinical Amoebiasis.The American journal of tropical medicine and hygiene · 2026
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  6. Colpodellosis: IsPathogens (Basel, Switzerland) · 2026
    Review
  7. Article
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  9. Taxonomy ofParasitology · 2026
    Review
  10. Stool Parasitological Examination: A Diagnostic Methodology Under Threat of Extinction.Revista da Sociedade Brasileira de Medicina Tropical · 2026
    Article
  11. Article
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  15. Review
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  17. Article
  18. Article
  19. Hemogregarines in bufonid anurans from the Brazilian Amazon, with description a new species ofInternational journal for parasitology. Parasites and wildlife · 2025
    Article
  20. Intestinal parasites infecting free-ranging primates in Colombia: Morphological and molecular evidence.International journal for parasitology. Parasites and wildlife · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 10 institutions in 6 countries.

Richard S BradburyFederation University, Melbourne, Australia.ORCID 0000-0002-5524-506X
Sarah G H SappDivision of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Idzi PottersInstitute of Tropical Medicine, Antwerp, Belgium.ORCID 0000-0001-7394-7033
Blaine A MathisonInstitute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, Utah, USA.ORCID 0000-0001-9975-941X
John FreanParasitology Reference Laboratory, National Institute for Communicable Diseases, Johannesburg, South Africa.
Abhishek MewaraDepartment of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Harsha SheoreyMicrobiology Department, Royal Melbourne Hospital, Melbourne, Australia.
Francesca TamarozziDepartment of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy.
Marc Roger CouturierInstitute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, Utah, USA.
Peter ChiodiniHospital for Tropical Diseases, London, United Kingdom.
Bobbi PrittDepartment of Laboratory Medicine and Pathology, Mayo Clinicgrid.66875.3a, Rochester, Minnesota, USA.ORCID 0000-0003-0261-1326
University of Utah · USCenters for Disease Control and Prevention · USFederation University · AUInstituut voor Tropische Geneeskunde · BELondon School of Hygiene & Tropical Medicine · GBMayo Clinic · USNational Institute for Communicable DiseasesOspedale Sacro Cuore Don Calabria · ITPost Graduate Institute of Medical Education and Research · INThe Royal Melbourne Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advances in laboratory techniques have revolutionized parasitology diagnostics over the past several decades. Widespread implementation of rapid antigen detection tests has greatly expanded access to tests for global parasitic threats such as malaria, while next-generation amplification and sequencing methods allow for sensitive and specific detection of human and animal parasites in complex specimen matrices. Recently, the introduction of multiplex panels for human gastrointestinal infections has enhanced the identification of common intestinal protozoa in feces along with bacterial and viral pathogens. Despite the benefits provided by novel diagnostics, increased reliance on nonmicroscopy-based methods has contributed to the progressive, widespread loss of morphology expertise for parasite identification. Loss of microscopy and morphology skills has the potential to negatively impact patient care, public health, and epidemiology. Molecular- and antigen-based diagnostics are not available for all parasites and may not be suitable for all specimen types and clinical settings. Furthermore, inadequate morphology experience may lead to missed and inaccurate diagnoses and erroneous descriptions of new human parasitic diseases. This commentary highlights the need to maintain expert microscopy and morphological parasitology diagnostic skills within the medical and scientific community. We proposed that light microscopy remains an important part of training and practice in the diagnosis of parasitic diseases and that efforts should be made to train the next generation of morphological parasitologists before the requisite knowledge, skills, and capacity for this complex and important mode of diagnosis are lost. In summary, the widespread, progressive loss of morphology expertise for parasite identification negatively impacts patient care, public health, and epidemiology.

Indexed as

ParasitesParasitic DiseasesAnimalsBacteriaFecesHumansMicroscopyarthropodscommunicable diseasesdiagnostic errorsdiagnosticsemergingepidemiologyhelminthshistologylaboratoriesmetagenomicsparasitesparasitic diseasesprotozoa

Identifiers

PMID36314793
PMCPMC9667774
OpenAlexW4307778506

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.