ArticleAngewandte Chemie (International ed. in English)2023
The Role of Cytochrome P450 AbyV in the Final Stages of Abyssomicin C Biosynthesis.
Article in Angewandte Chemie (International ed. in English), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 15 citations in OpenAlex.
- Delineation of the complete reaction cycle of a natural Diels-Alderase.Chemical science · 2024Article
- Discovery and biosynthesis of bacterial drimane-type sesquiterpenoids fromBeilstein journal of organic chemistry · 2024Article
- β-Lactams from the Ocean.Marine drugs · 2023Review
- The Role of Cytochrome P450 AbyV in the Final Stages of Abyssomicin C Biosynthesis.Angewandte Chemie (International ed. in English) · 2023Article
- The Role of Cytochrome P450 AbyV in the Final Stages of Abyssomicin C Biosynthesis.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
Abyssomicin C and its atropisomer are potent inhibitors of bacterial folate metabolism. They possess complex polycyclic structures, and their biosynthesis has been shown to involve several unusual enzymatic transformations. Using a combination of synthesis and in vitro assays we reveal that AbyV, a cytochrome P450 enzyme from the aby gene cluster, catalyses a key late-stage epoxidation required for the installation of the characteristic ether-bridged core of abyssomicin C. The X-ray crystal structure of AbyV has been determined, which in combination with molecular dynamics simulations provides a structural framework for our functional data. This work demonstrates the power of combining selective carbon-13 labelling with NMR spectroscopy as a sensitive tool to interrogate enzyme-catalysed reactions in vitro with no need for purification.
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Registered trials
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