Evidence map›Paper›PMID 36313809›Full record

ArticleCell reports methods2022

Heterologous reporter expression in the planarian

Richard Nelson Hall, Uri Weill, Leonard Drees, Sergio Leal-Ortiz, Hongquan Li, Margarita Khariton, Chew Chai, Yuan Xue, Benyamin Rosental, Stephen R Quake and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 17 citations in OpenAlex.

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  14. mRNA Transfection of S. mediterranea for Luminescence Analysis.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 3 countries.

Richard Nelson HallDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Uri WeillDepartment of Tissue Dynamics and Regeneration, Max Planck Institute for Multidisciplinary Sciences, Göttingen 37077, Germany.
Leonard DreesDepartment of Tissue Dynamics and Regeneration, Max Planck Institute for Multidisciplinary Sciences, Göttingen 37077, Germany.
Sergio Leal-OrtizDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA.
Hongquan LiDepartment of Electrical Engineering, Stanford University, Stanford, CA 94305, USA.
Margarita KharitonDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Chew ChaiDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Yuan XueDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Benyamin RosentalThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer-Sheva 84105, Israel.
Stephen R QuakeDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Alejandro Sánchez AlvaradoStowers Institute for Medical Research, Kansas City, MO 64110, USA.
Nicholas A MeloshDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA.
Andrew Z FireDepartment of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Jochen C RinkDepartment of Tissue Dynamics and Regeneration, Max Planck Institute for Multidisciplinary Sciences, Göttingen 37077, Germany.
Bo WangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Stanford University · USMax Planck Institute for Dynamics and Self-Organization · DEBen-Gurion University of the Negev · ILChan Zuckerberg Initiative (United States) · USStowers Institute for Medical Research · US

Funding

Cellular Response to Genetic ChangeR35GM130366 · NIGMS · STANFORD UNIVERSITY · PI ANDREW Z. FIRE · 2019 to 2026
$4.8M
NIGMS NIH HHS R35 GM130366
6 · The paper itself

Abstract

Planarians have long been studied for their regenerative abilities. Moving forward, tools for ectopic expression of non-native proteins will be of substantial value. Using a luminescent reporter to overcome the strong autofluorescence of planarian tissues, we demonstrate heterologous protein expression in planarian cells and live animals. Our approach is based on the introduction of mRNA through several nanotechnological and chemical transfection methods. We improve reporter expression by altering untranslated region (UTR) sequences and codon bias, facilitating the measurement of expression kinetics in both isolated cells and whole planarians using luminescence imaging

Indexed as

PlanariansAnimalsMediterraneaModels, BiologicalRNA, MessengerTransfectionRNA, Messengerflatwormgene deliveryluminescencemRNA transfectionplanarianpost-transcriptional regulationSchmidtea mediterraneasomatic transformation

Identifiers

PMID36313809
PMCPMC9606109
OpenAlexW4297859497

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.