ArticleFrontiers in cell and developmental biology2022
Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows.
Article in Frontiers in cell and developmental biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution.Nature metabolism · 2025Guideline
- Angiogenic behavior of human umbilical vein endothelial cells in hydrogel containing lyophilized powder of decellularized adipose tissue from high-fat diet-fed or exercise-trained rats.Metabolism open · 2026Article
- Understanding adipocyte heterogeneity across species, depot, and disease.Biochemical Society transactions · 2026Review
- Transcriptional Adaptations to Muscle Loading in a Murine Model of Achilles Tendinopathy.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- CD13 is a bona-fide marker of bovine pre-adipocytes with potential in cultivated fat applications.NPJ science of food · 2026Article
- Nuclei isolation from rat and cow white adipose tissues for single-nucleus RNA sequencing; rat WAT remains a challenge.Frontiers in physiology · 2026Article
- Article
- Depot-dependent effects of subclinical ketosis on visceral and subcutaneous adipose tissue transcriptional cellular diversity in dairy cows.Journal of animal science and biotechnology · 2025Article
- Article
- Multi-omics integrated analysis reveals the molecular mechanism of tail fat deposition differences in sheep with different tail types.BMC genomics · 2025Article
- Subclinical ketosis in postpartum dairy cows alters the adipose tissue immunological profile in a depot-specific manner.Frontiers in immunology · 2025Article
- Key questions and gaps in understanding adipose tissue macrophages and early-life metabolic programming.American journal of physiology. Endocrinology and metabolism · 2024Review
- The Effect of Subclinical Ketosis on the Peripheral Blood Mononuclear Cell Inflammatory Response and Its Crosstalk with Depot-Specific Preadipocyte Function in Dairy Cows.Animals : an open access journal from MDPI · 2024Article
- Single-Cell Sequencing Technology in Ruminant Livestock: Challenges and Opportunities.Current issues in molecular biology · 2024Review
- A cell atlas of thoracic aortic perivascular adipose tissue: a focus on mechanotransducers.American journal of physiology. Heart and circulatory physiology · 2024Article
- Inflammatory tone in liver and adipose tissue in dairy cows experiencing a healthy transition from late pregnancy to early lactation.Journal of dairy science · 2023Article
- A Cell Atlas of Thoracic Aortic Perivascular Adipose Tissue: a focus on mechanotransducers.bioRxiv : the preprint server for biology · 2023Article
- White Adipose Tissue Heterogeneity in the Single-Cell Era: From Mice and Humans to Cattle.Biology · 2023Review
- Optimized nucleus isolation protocol from frozen mouse tissues for single nucleus RNA sequencing application.Frontiers in cell and developmental biology · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adipose tissue (AT) is an endocrine organ with a central role on whole-body energy metabolism and development of metabolic diseases. Single-cell and single-nuclei RNA sequencing (scRNA-seq and snRNA-seq, respectively) analyses in mice and human AT have revealed vast cell heterogeneity and functionally distinct subtypes that are potential therapeutic targets to metabolic disease. In periparturient dairy cows, AT goes through intensive remodeling and its dysfunction is associated with metabolic disease pathogenesis and decreased productive performance. The contributions of depot-specific cells and subtypes to the development of diseases in dairy cows remain to be studied. Our objective was to elucidate differences in cellular diversity of visceral (VAT) and subcutaneous (SAT) AT in dairy cows at the single-nuclei level. We collected matched SAT and VAT samples from three dairy cows and performed snRNA-seq analysis. We identified distinct cell types including four major mature adipocytes (AD) and three stem and progenitor cells (ASPC) subtypes, along with endothelial cells (EC), mesothelial cells (ME), immune cells, and pericytes and smooth muscle cells. All major cell types were present in both SAT and VAT, although a strong VAT-specificity was observed for ME, which were basically absent in SAT. One ASPC subtype was defined as adipogenic (
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