Evidence map›Paper›PMID 36313539›Full record

ArticleSTAR protocols2022

A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry.

Zhengyuan Pang, Li Ye

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Zhengyuan PangDepartment of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address: zypang@scripps.edu.
Li YeDepartment of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address: liye@scripps.edu.

Funding

Modulating somatosensory network to target metabolic diseasesDP2DK128800 · NIDDK · SCRIPPS RESEARCH INSTITUTE, THE · PI YE, LI · 2020 to 2020
$2.7M
Feeding regulation by cortical-amygdala circuitsR03DK124731 · NIDDK · SCRIPPS RESEARCH INSTITUTE, THE · PI YE, LI · 2020 to 2021
$266k
NIDDK NIH HHS DP2 DK128800NIDDK NIH HHS R03 DK124731
6 · The paper itself

Abstract

Here, we provide a protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution. By combining tissue clearing and click chemistry, this protocol allows fluorescence tagging of covalent drug binding

Indexed as

Click ChemistryMammalsAnimalsStaining and LabelingBiotechnology and bioengineeringNeuroscience

Identifiers

PMID36313539
PMCPMC9615031

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.