Evidence map›Paper›PMID 36313201›Full record

ArticleBio-protocol2022

Extraction and Quantification of Plant Hormones and RNA from Pea Axillary Buds.

Da Cao, Francois Barbier, Kaori Yoneyama, Christine A Beveridge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Da CaoARC Centre of Excellence for Plant Success in Nature and Agriculture, School of Biological Sciences, The University of Queensland, St Lucia, 4072, Australia.
Francois BarbierARC Centre of Excellence for Plant Success in Nature and Agriculture, School of Biological Sciences, The University of Queensland, St Lucia, 4072, Australia.
Kaori YoneyamaGraduate School of Agriculture, Ehime University, Matsuyama, Japan.
Christine A BeveridgeARC Centre of Excellence for Plant Success in Nature and Agriculture, School of Biological Sciences, The University of Queensland, St Lucia, 4072, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The quantification of plant hormones and related gene expression is essential to improve the understanding of the molecular regulation of plant growth and development. However, plant hormone quantification is still challenging due to extremely low endogenous levels and high chemical diversity. In this study, we present a convenient extraction protocol that enables the simultaneous extraction of both phytohormones and RNA from the same sample in a small quantity (approximately 10 mg). Using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS), this protocol provides a method to quantify 13 phytohormones and their derivatives from four classes (cytokinin, auxin, abscisic acid, and gibberellin) at the speed of 14 min per sample.

Indexed as

Abscisic acidAuxinCytokininGibberellinPea axillary budsPhytohormone and RNA extractionPhytohormone quantificationUPLC–MS/MS

Identifiers

PMID36313201
PMCPMC9548514

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.