ArticleFrontiers in plant science2022
Characterization of complex photosynthetic pigment profiles in European deciduous tree leaves by sequential extraction and reversed-phase high-performance liquid chromatography.
Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed.
- Chorismate and isochorismate turnover reveal hidden dynamics of aromatic metabolism in Arabidopsis leaves.The Plant cell · 2026Article
- Tomato Leaf Color Diversity as a Functional Trait: Molecular Mechanisms, Physiological Significance, and Environmental Responses.International journal of molecular sciences · 2026Review
- Foliar application of microbial consortium formulations suppresses Phomopsis leaf blight, promotes vegetative growth, and modulates biochemical defence in strawberry (Frontiers in plant science · 2026Article
- Isolation of highly polar galloyl glucoside tautomers from Saxifraga tangutica through preparative chromatography and assessment of their in vitro antioxidant activity.BMC chemistry · 2024Article
- Inoculation withPlants (Basel, Switzerland) · 2023Article
- Late-season biosynthesis of leaf fatty acids andFrontiers in plant science · 2022Article
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Authors and funding
2 authors.
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Abstract
Leaf pigments, including chlorophylls and carotenoids, are important biochemical indicators of plant photosynthesis and photoprotection. In this study, we developed, optimized, and validated a sequential extraction and liquid chromatography-diode array detection method allowing for the simultaneous quantification of the main photosynthetic pigments, including chlorophyll a, chlorophyll b, β-carotene, lutein, neoxanthin, and the xanthophyll cycle (VAZ), as well as the characterization of plant pigment derivatives. Chromatographic separation was accomplished with the newest generation of core-shell columns revealing numerous pigment derivatives. The sequential extraction allowed for a better recovery of the main pigments (+25 % chlorophyll a, +30 % chlorophyll b, +42 % β-carotene, and 61% xanthophylls), and the characterization of ca. 5.3 times more pigment derivatives (i.e., up to 62 chlorophyll and carotenoid derivatives including isomers) than with a single-step extraction. A broad working range of concentrations (300-2,000 ng.mL
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