Evidence map›Paper›PMID 36310226›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

In Vivo Analysis of a Biomolecular Condensate in the Nervous System of C. elegans.

Matthew G Andrusiak, Yishi Jin

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 69% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Mapping SARS-CoV-2 Nucleocapsid Function with Nanobodies.bioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Matthew G AndrusiakDepartment of Neurobiology, School of Biological Sciences, University of California, San Diego, CA, USA.
Yishi JinDepartment of Neurobiology, School of Biological Sciences, University of California, San Diego, CA, USA. yijin@ucsd.edu.
University of California, San Diego · US

Funding

Cytoskeletal dynamics in axon regenerationR01NS093588 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHISHOLM, ANDREW D, JIN, YISHI · 2015 to 2023
$3.7M
GABAergic Neuron Differentiation in C.elegansR37NS035546 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JIN, YISHI · 2017 to 2021
$2.4M
CIHR MFE-146808NINDS NIH HHS R01 NS093588NINDS NIH HHS R37 NS035546
6 · The paper itself

Abstract

Liquid-liquid phase separation (LLPS) has emerged as a common biophysical event that facilitates the formation of non-membrane-bound cellular compartments, also termed biomolecular condensates. Since the first report of a biomolecular condensate in the germline of C. elegans, many regulatory hubs have been shown to have similar liquid-like features. With the wealth of molecules now being reported to possess liquid-like features, an impetus has been placed on reconciling LLPS with regulation of specific biological properties in vivo. Herein, we report a methodology used to study LLPS-associated features in C. elegans neurons, illustrated using the RNA granule protein TIAR-2. In axons, TIAR-2 forms liquid-like granules, which following injury are inhibitory to the regeneration process. Measuring the dynamics of TIAR-2 granules provides a tractable biological output to study LLPS function. In conjunction with other established methods to assess LLPS, the results from the protocol outlined provide comprehensive insight regarding this important biophysical property.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsAnimalsAxonsBiomolecular CondensatesGerm CellsCaenorhabditis elegans ProteinsAxon injuryAxon regenerationBiomolecular condensateLiquid–liquid phase separationProtein dynamicsRNA granulesStress granulesTIAR-2

Identifiers

PMID36310226
OpenAlexW4307642788

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.