Mapping Phase Diagram of Tau-RNA LLPS Under Live Cell Coculturing Conditions.

Yanxian Lin, Yann Fichou, Jennifer N Rauch, Xuemei Zhang, Kenneth S Kosik, Songi Han

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yanxian LinBiomolecular Science and Engineering, University of California, Santa Barbara, CA, USA.

Yann FichouDepartment of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA.

Jennifer N RauchMolecular, Cell and Developmental Biology, University of California, Santa Barbara, CA, USA.

Xuemei ZhangNeuroscience Research Institute, University of California, Santa Barbara, CA, USA.

Kenneth S KosikMolecular, Cell and Developmental Biology, University of California, Santa Barbara, CA, USA.

Songi HanDepartment of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA. songihan@ucsb.edu.

Funding

Molecular Basis of the Tau Aggregation PathwayR01AG056058 · NIA · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Songi Han, KENNETH Stephen KOSIK · 2017 to 2026

$6.4M

NIA NIH HHS R01 AG056058

6 · The paper itself

Abstract

Protein liquid-liquid phase separation (LLPS) has been associated with biological functions and pathological aggregation. Mapping the phase separation conditions is the first step to identify and quantify the driving forces of LLPS. Here, we describe the protocols to draw the phase diagram of tau-RNA LLPS and use the mapped diagram to guide experimental conditions for LLPS-cell coculturing, electron resonance spectroscopy in particular double electron-electron resonance spectroscopy, crosslinking immunoprecipitation, and isothermal titration calorimetry.

Indexed as

RNARNAComplex coacervationDouble electron-electron resonanceLiquid-liquid phase separationPhase diagramTau protein

Identifiers

PMID36310209

DOI10.1007/978-1-0716-2597-2_18

PMCPMC13002136

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.