Evidence map›Paper›PMID 36308684›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Enhanced Proteomic Data Analysis with MetaMorpheus.

Rachel M Miller, Robert J Millikin, Zach Rolfs, Michael R Shortreed, Lloyd M Smith

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Immunopeptidomics in the cancer immunotherapy era.Exploration of targeted anti-tumor therapy · 2024
    Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rachel M MillerUniversity of Wisconsin-Madison, Department of Chemistry, Madison, WI, USA.
Robert J MillikinUniversity of Wisconsin-Madison, Department of Chemistry, Madison, WI, USA.
Zach RolfsUniversity of Wisconsin-Madison, Department of Chemistry, Madison, WI, USA.
Michael R ShortreedUniversity of Wisconsin-Madison, Department of Chemistry, Madison, WI, USA.
Lloyd M SmithUniversity of Wisconsin-Madison, Department of Chemistry, Madison, WI, USA. smith@chem.wisc.edu.

Funding

Institutional Training in the Genomic SciencesT32HG002760 · NHGRI · UNIVERSITY OF WISCONSIN-MADISON · PI Qiongshi Lu · 2003 to 2026
$17.7M
Chemistry-Biology Interface Training ProgramT32GM008505 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BLACKWELL, HELEN E. · 1993 to 2023
$9.8M
Revealing Proteoforms: The Primary Effectors of Biological FunctionR35GM126914 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Lloyd M Smith · 2018 to 2026
$6.2M
Expanding multiplexed kinase biosensor analysis to SWATH-MS (Part 2 of 2)U24CA199347 · NCI · UNIVERSITY OF MINNESOTA · PI GRIFFIN, TIMOTHY J. · 2016 to 2019
$2.4M
NCI NIH HHS U24 CA199347NHGRI NIH HHS T32 HG002760NIGMS NIH HHS R35 GM126914NIGMS NIH HHS T32 GM008505
6 · The paper itself

Abstract

MetaMorpheus is a free and open-source software program dedicated to the comprehensive analysis of proteomic data. In bottom-up proteomics, protein samples are digested into peptides prior to chromatographic separation and tandem mass spectrometric analysis. The resulting fragmentation spectra are subsequently analyzed with search software programs to obtain peptide identifications and infer the presence of proteins in the samples. MetaMorpheus seeks to maximize the information gleaned from proteomic data through the use of (a) mass calibration, (b) post-translational modification discovery, (c) multiple search algorithms, which aid in the analysis of data from traditional, crosslinking, and glycoproteomic experiments, (d) isotope-based or label-free quantification, (e) multi-protease protein inference, and (f) spectral annotation and data visualization capabilities. This protocol provides detailed descriptions of how use MetaMorpheus and how to customize data analysis workflows using MetaMorpheus tasks to meet the specific needs of the user.

Indexed as

Data AnalysisProteomicsAlgorithmsDatabases, ProteinPeptidesProteinsSoftwareTandem Mass SpectrometryPeptidesProteinsBottom-upCrosslinkDatabase searchGlycopeptidesOpen-sourcePost-translational modification discoveryProteomicsTandem mass spectrometry

Identifiers

PMID36308684
PMCPMC9623450

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.