Evidence map›Paper›PMID 36307807›Full record

ArticleMicrobial cell factories2022

A facile process for adipic acid production in high yield by oxidation of 1,6-hexanediol using the resting cells of Gluconobacter oxydans.

Sang-Hyun Pyo, Mahmoud Sayed, Oliver Englund Örn, Jorge Amorrortu Gallo, Nídia Fernandez Ros, Rajni Hatti-Kaul

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Sang-Hyun PyoDivision of Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, 22100, Lund, Sweden. Sang-Hyun.Pyo@biotek.lu.se.
Mahmoud SayedDivision of Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, 22100, Lund, Sweden.
Oliver Englund ÖrnDivision of Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, 22100, Lund, Sweden.
Jorge Amorrortu Gallo *Division of Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, 22100, Lund, Sweden.
Nídia Fernandez Ros *Division of Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, 22100, Lund, Sweden.
Rajni Hatti-KaulDivision of Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, 22100, Lund, Sweden.
Lund University · SE

Funding

Stiftelsen för Miljöstrategisk Forskning 2016/1489
6 · The paper itself

Abstract

backgroundAdipic acid (AA) is one of the most important industrial chemicals used mainly for the production of Nylon 6,6 but also for making polyurethanes, plasticizers, and unsaturated polyester resins, and more recently as a component in the biodegradable polyester poly(butylene adipate terephthalate) (PBAT). The main route for AA production utilizes benzene as feedstock and generates copious amounts of the greenhouse gas NO

resultsThe present report involves a study on the effect of different parameters on the microbial transformation of 1,6-hexanediol to adipic acid, and subsequently testing the process on a larger lab scale for achieving maximal conversion and yield. Comparison of three wild-type strains of G. oxydans DSM50049, DSM2003, and DSM2343 for the whole-cell biotransformation of 10 g/L 1,6-hexanediol to adipic acid in batch mode at pH 7 and 30 °C led to the selection of G. oxydans DSM50049, which showed 100% conversion of the substrate with over 99% yield of adipic acid in 30 h. An increase in the concentrations of the substrate decreased the degree of conversion, while the product up to 25 g/L in batch and 40 g/L in fed-batch showed no inhibition on the conversion. Moreover, controlling the pH of the reaction at 5-5.5 was required for the cascade oxidation reactions to work. Cell recycling for the biotransformation resulted in a significant decrease in activity during the third cycle. Meanwhile, the fed-batch mode of transformation by intermittent addition of 1,6-hexanediol (30 g in total) in 1 L scale resulted in complete conversion with over 99% yield of adipic acid (approximately 37 g/L). The product was recovered in a pure form using downstream steps without the use of any solvent.

conclusionA facile, efficient microbial process for oxidation of 1,6-hexanediol to adipic acid, having potential for scale up was demonstrated. The entire process is performed in aqueous medium at ambient temperatures with minimal greenhouse gas emissions. The enzymes involved in catalyzing the oxidation steps are currently being identified.

Indexed as

Gluconobacter oxydansGreenhouse GasesAdipatesGlycolsPolyestersAdipatesadipic acidGlycolsGreenhouse Gaseshexamethylene glycolPolyesters1,6-hexanediolAdipic acidGluconobacter oxidationPolymer building blockWhole cell oxidation

Identifiers

PMID36307807
PMCPMC9617331
OpenAlexW4307840960

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.