ArticleApplied microbiology and biotechnology2022
Enhancing xylanase expression by Komagataella phaffii by formate as carbon source and inducer.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- FormatotrophicSynthetic and systems biotechnology · 2026Article
- Recent progress in the microbial production of Xylanase.World journal of microbiology & biotechnology · 2025Review
- Sorbitol Uptake and Oxygen Transfer Shape AOX1 Promoter Induction in Formate Dehydrogenase-Deficient Komagataella phaffii.Microbial biotechnology · 2025Article
- Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges.FEMS yeast research · 2025Review
- Development of a Komagataella phaffii cell factory for sustainable production of ( +)-valencene.Microbial cell factories · 2025Article
- Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficient Komagataella phaffii.Microbial biotechnology · 2024Article
- Advances in Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Review
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4 authors at 1 institution in 1 country.
Funding
Abstract
Komagataella phaffii (syn. Pichia pastoris), a methylotrophic yeast, has many advantages as a protein expression system, but has the disadvantage of hazardous methanol as an inducer and carbon source. To enable substitution of formate for methanol, a formate assimilation pathway was constructed by the co-expression of acetyl-CoA synthase, acetaldehyde dehydrogenase, and transcription factor Mit1, resulting in a 103.5 ± 12.5% increase in xylanase production. Recombinant K. phaffii was able to use formate as a carbon source, indicating successful substitution of formate for methanol. Xylanase production, using the safe and sustainable formate as an inducer and carbon source, is a major advance in the field of industrial enzyme production. KEY POINTS: • Change to formate assimilation by recombinant K. phaffii instead of methanol • K. phaffii expressed xylanase by formate induction instead of methanol induction • Increased xylanase expression by transcription factor co-expression.
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