Evidence map›Paper›PMID 36307466›Full record

ArticleScientific reports2022

Transcriptome analysis of mulberry (Morus alba L.) leaves to identify differentially expressed genes associated with post-harvest shelf-life elongation.

Dipayan Das, Subires Bhattacharyya, Monidipa Bhattacharyya, Puja Sashankar, Arindam Ghosh, Palash Mandal

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Dipayan DasPlant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri, West Bengal, 734013, India.
Subires BhattacharyyaPlant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri, West Bengal, 734013, India.
Monidipa BhattacharyyaPlant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri, West Bengal, 734013, India.
Puja Sashankar *Department of Botany, Government General Degree College, Gorubathan, Fagu, Kalimpong, 735231, India.
Arindam Ghosh *Centre of Bioinformatics, Institute of Interdisciplinary Studies, University of Allahabad, Prayagraj, 211002, India.
Palash MandalPlant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri, West Bengal, 734013, India. pmandalbotppprl@nbu.ac.in.
North Bengal University · INUniversity of Allahabad · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Present study deals with molecular expression patterns responsible for post-harvest shelf-life extension of mulberry leaves. Quantitative profiling showed retention of primary metabolite and accumulation of stress markers in NS7 and CO7 respectively. The leaf mRNA profiles was sequenced using the Illumina platform to identify DEGs. A total of 3413 DEGs were identified between the treatments. Annotation with Arabidopsis database has identified 1022 DEGs unigenes. STRING generated protein-protein interaction, identified 1013 DEGs nodes with p < 1.0e-16. KEGG classifier has identified genes and their participating biological processes. MCODE and BiNGO detected sub-networking and ontological enrichment, respectively at p ≤ 0.05. Genes associated with chloroplast architecture, photosynthesis, detoxifying ROS and RCS, and innate-immune response were significantly up-regulated, responsible for extending shelf-life in NS7. Loss of storage sucrose, enhanced activity of senescence-related hormones, accumulation of xenobiotics, and development of osmotic stress inside tissue system was the probable reason for tissue deterioration in CO7. qPCR validation of DEGs was in good agreement with RNA sequencing results, indicating the reliability of the sequencing platform. Present outcome provides a molecular insight regarding involvement of genes in self-life extension, which might help the sericulture industry to overcome their pre-existing problems related to landless farmers and larval feeding during monsoon.

Indexed as

MorusGene Expression ProfilingGene Expression Regulation, PlantPlant LeavesReproducibility of ResultsSequence Analysis, RNATranscriptome

Identifiers

PMID36307466
PMCPMC9616847
OpenAlexW4307841909

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.