Evidence map›Paper›PMID 36306076›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies.

Jacqueline Hollensteiner, Franziska Wemheuer, Dominik Schneider, Birgit Pfeiffer, Bernd Wemheuer

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jacqueline HollensteinerInstitute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany.
Franziska WemheuerInstitute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany.
Dominik SchneiderInstitute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany.
Birgit PfeifferInstitute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany.
Bernd WemheuerInstitute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany. bwemheu@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microbial communities play an important role in marine ecosystem processes. Although the number of studies targeting marker genes such as the 16S rRNA gene has increased during the last years, the vast majority of marine diversity are rather unexplored. Moreover, most studies focused on the entire microbial community and thus do not assess the active fraction of the microbial community. Here, we describe a detailed protocol for the simultaneous extraction of DNA and RNA from marine water samples and the generation of cDNA from the isolated RNA that can be used as a universal template in various marker gene studies.

Indexed as

DNAMicrobiotaDNA, ComplementaryMetagenomicsPhylogenyRNA, Ribosomal, 16SSequence Analysis, DNADNADNA, ComplementaryRNA, Ribosomal, 16SMarker gene studiesMetagenomicsMetatranscriptomicsMicrobial diversityMicrobial functions

Identifiers

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.