ArticleArtificial organs2023
Weight gain is an early indicator of injury in ex vivo normothermic limb perfusion (EVNLP).
Article in Artificial organs, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 28 citations in OpenAlex.
- Integrating Dialysis in Ex Situ Machine Perfusion: A Systematic Review and Meta-Analysis of Outcomes.Artificial organs · 2026Pooled it
- A meta-analysis of perfusion parameters affecting weight gain in ex vivo perfusion.Artificial organs · 2025Pooled it
- Dual acellular subnormothermic machine perfusion for enhanced uterus preservation in a swine model.Acta obstetricia et gynecologica Scandinavica · 2026Article
- Acellular Normothermic Machine Perfusion Does not Exacerbate Acute Rejection in Rodent Vascularized Composite Allografts.Transplantation direct · 2026Article
- Development of a Swine Whole Eye Transplant Ex Vivo Perfusion Protocol.Artificial organs · 2026Article
- 24-hourFrontiers in transplantation · 2026Article
- Time as a Therapeutic Ally: The Promise of Long-Term Solid Organ and Tissue Perfusion.Transplant international : official journal of the European Society for Organ Transplantation · 2026Review
- Ex-vivo limb perfusion in military and civilian medicine: inspired by ex-vivo organ perfusion, pioneered for traumatic limb amputation and peripheral nerve regeneration.Military Medical Research · 2025Article
- Development of a 24-h Preservation Protocol of Forearm Vascularized Composite Allotransplants in Nonhuman Primates Using Subnormothermic Machine Perfusion.Transplantation direct · 2025Article
- Ex Vivo Preservation of Porcine Vascularized Composite Soft Tissue Allografts.Artificial organs · 2025Article
- A Guide to the Implementation and Design of Ex Vivo Perfusion Machines for Vascularized Composite Allotransplantation.Plastic and reconstructive surgery. Global open · 2025Article
- Vascular Microphysiological System for Investigating Endothelial Barrier Function During Organ Preservation and Reperfusion.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Clinical study on basal blood perfusion in the major arteries of the limbs.Frontiers in medicine · 2025Article
- [Advances and perspectives in vascularized composite allotransplantation preservation].Bulletin de l'Academie nationale de medecine · 2024Article
- Enhanced VCA Storage: A Pilot Study Demonstrating Supercooling in Orthotopic Rodent Hindlimb Transplantation.Transplantation proceedings · 2024Article
- Sub-zero non-freezing of vascularized composite allografts in a rodent partial hindlimb model.Cryobiology · 2024Article
- VCA supercooling in a swine partial hindlimb model.Scientific reports · 2024Article
- Towards Optimizing Sub-Normothermic Machine Perfusion in Fasciocutaneous Flaps: A Large Animal Study.Bioengineering (Basel, Switzerland) · 2023Article
- Weight gain is an early indicator of injury in ex vivo normothermic limb perfusion (EVNLP).Artificial organs · 2023Article
- Review of machine perfusion studies in vascularized composite allotransplant preservation.Frontiers in transplantation · 2023Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
purposeThere are no established criteria for discontinuing ex vivo normothermic limb perfusion (EVNLP) before irreversible damage occurs. This study evaluates weight gain as an indicator of injury during EVNLP.
methodsSixteen Yorkshire pig forelimbs were procured and preserved using EVNLP with a hemoglobin-based oxygen carrier (HBOC-201) or static cold storage. EVNLP continued until termination criteria were met: arterial pressure ≥ 115 mm Hg, compartment pressure > 30 mm Hg, or 20% reduction of oxygen saturation. Limb weight, contractility, hemodynamics, perfusate electrolytes, metabolites and gases were recorded. Muscles were biopsied 6-h, and muscle injury scores (MIS) calculated. Forearm compartment pressures and indocyanine green (ICG) angiography were recorded at endpoint. Outcomes were compared at 2%, 5%, 10%, and 20% limb weight gain.
resultsEVNLP lasted 20 ± 3 h. Weight gain was observed after 13 ± 5 h (2%), 15 ± 6 h (5%), 16 ± 6 h (10%), and 19 ± 4 h (20%). Weight correlated positively with MIS (ρ = 0.92, p < 0.0001), potassium (ρ = -1.00, p < 0.0001), pressure (ρ = 0.78, p < 0.0001), and negatively with contractility (ρ = -0.96, p = 0.011). At 5% weight gain, MIS (p < 0.0001), potassium (p = 0.03), and lactate (p < 0.0001) were significantly higher than baseline. Median muscle contractility was 5 [3-5] at 2% weight gain, 4 [1-5] at 5%, 3 [0-4] and 2 [0-2] at 10% and 20%, respectively. At 20% weight gain, contractility was significantly lower than baseline (p = 0.003). Percent weight gain correlated negatively with endpoint ICG hoof fluorescence (r = -0.712, p = 0.047).
conclusionsWeight gain correlated with microscopic muscle injury and was the earliest evidence of limb dysfunction. Weight gain may serve as a criterion for discontinuation of EVNLP.
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