ArticleaBIOTECH2020
A novel CRISPR/Cas9 system for efficiently generating Cas9-free multiplex mutants in
Article in aBIOTECH, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- The signaling mechanism of phyA involves direct interaction with ATG8 to regulate HY5 autophagic degradation under nutrient starvation.The New phytologist · 2026Article
- A redox switch on CDC48 regulates peroxisomal function via controlling peroxisome-associated protein degradation.Nature communications · 2026Article
- The BZR1-BLH1-PIF4 feedback module acts as a molecular amplifier for precise thermomorphogenesis in Arabidopsis.Nature communications · 2026Article
- Article
- Arabidopsis IONIC CURRENT FAMILY A proteins facilitate environmental calcium acquisition essential for stress tolerance.Nature plants · 2026Article
- Transgenic gamete cleaning system enabled high-throughput screening for non-transgenic gene-edited plants.Functional & integrative genomics · 2025Article
- The peanutaBIOTECH · 2025Article
- Development of an RNA aptamer-assisted CRISPR/Cas9 system for efficiently generating and isolating Cas9-free mutants in plant.PLoS genetics · 2025Article
- A homeobox transcription factor HB34 suppresses jasmonic acid biosynthesis but promotes the expression of growth-related genes to balance plant immunity and growth in Arabidopsis.Plant communications · 2025Article
- CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- A thermosensor FUST1 primes heat-induced stress granule formation via biomolecular condensation in Arabidopsis.Cell research · 2025Article
- DSK2-mediated degradation of F-box protein LAO1 and class I TCPs modulates the nitrogen starvation response.EMBO reports · 2025Article
- The apoplastic pH is a key determinant in the hypocotyl growth response to auxin dosage and light.Nature plants · 2025Article
- Modern Plant Breeding Techniques in Crop Improvement and Genetic Diversity: From Molecular Markers and Gene Editing to Artificial Intelligence-A Critical Review.Plants (Basel, Switzerland) · 2024Review
- Arabidopsis transcription factor TCP4 controls the identity of the apical gynoecium.The Plant cell · 2024Article
- A CRISPR/Cas9-induced male-sterile line facilitating easy hybrid production in polyploid rapeseed (Horticulture research · 2024Article
- A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection ofaBIOTECH · 2024Article
- The Role of FveAFB5 in Auxin-Mediated Responses and Growth in Strawberries.Plants (Basel, Switzerland) · 2024Article
- Gene Stacking and Stoichiometric Expression of ER-Targeted Constructs Using "2A" Self-Cleaving Peptides.Methods in molecular biology (Clifton, N.J.) · 2024Article
- CRISPR/Cas9-gene editing approaches in plant breeding.GM crops & food · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 genome-editing system has emerged as a popular powerful tool for biological research. However, the process of selecting efficiently edited Cas9-free plants is usually laborious and time consuming. Here, we demonstrated P2A to be the most efficient self-cleaving peptide for fusing Cas9 and GFP in
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.