ReviewaBIOTECH2020
Precise gene replacement in plants through CRISPR/Cas genome editing technology: current status and future perspectives.
Review in aBIOTECH, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Genome Editing Approaches in Flax (International journal of molecular sciences · 2026Review
- Molecular characterization of humanized APOE mouse models reveals source and genotype dependent differences.Molecular neurodegeneration · 2026Article
- Advances in plant metabolic engineering through synthetic biology.Frontiers in plant science · 2026Review
- Bridging scales: integrated multi-omics and deep phenotyping for climate resilience in crop plants.Frontiers in plant science · 2026Review
- Reinforced Defenses:International journal of molecular sciences · 2025Review
- Article
- Manipulating brassinosteroid signaling pathway to genetically improve horticultural plants.aBIOTECH · 2025Review
- Isolation and Transfection of Nicotiana benthamiana Mesophyll Protoplasts.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- CRISPR-Cas technology secures sustainability through its applications: a review in green biotechnology.3 Biotech · 2023Review
- The economics and policy of genome editing in crop improvement.The plant genome · 2023Review
- CRISPR/Cas9-Mediated Targeted DNA Integration: Rearrangements at the Junction of Plant and Plasmid DNA.International journal of molecular sciences · 2022Article
- CRISPR-Cas technology a new era in genomic engineering.Biotechnology reports (Amsterdam, Netherlands) · 2022Review
- An update on precision genome editing by homology-directed repair in plants.Plant physiology · 2022Article
- An Efficient Marker Gene Excision Strategy Based on CRISPR/Cas9-Mediated Homology-Directed Repair in Rice.International journal of molecular sciences · 2022Article
- CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects.Frontiers in genetics · 2022Review
- Recent advances in CRISPR/Cas9 and applications for wheat functional genomics and breeding.aBIOTECH · 2021Review
- Gene Targeting Facilitated by Engineered Sequence-Specific Nucleases: Potential Applications for Crop Improvement.Plant & cell physiology · 2021Review
- Frontiers in the Standardization of the Plant Platform for High Scale Production of Vaccines.Plants (Basel, Switzerland) · 2021Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas, as a simple, versatile, robust and cost-effective system for genome manipulation, has dominated the genome editing field over the past few years. The application of CRISPR/Cas in crop improvement is particularly important in the context of global climate change, as well as diverse agricultural, environmental and ecological challenges. Various CRISPR/Cas toolboxes have been developed and allow for targeted mutagenesis at specific genome loci, transcriptome regulation and epigenome editing, base editing, and precise targeted gene/allele replacement or tagging in plants. In particular, precise replacement of an existing allele with an elite allele in a commercial variety through homology-directed repair (HDR) is a holy grail in genome editing for crop improvement as it has been very difficult, laborious and time-consuming to introgress the elite alleles into commercial varieties without any linkage drag from parental lines within a few generations in crop breeding practice. However, it still remains very challenging in crop plants. This review intends to provide an informative summary of the latest development and breakthroughs in gene replacement using CRISPR/Cas technology, with a focus on achievements, potential mechanisms and future perspectives in plant biological science as well as crop improvement.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.