ArticleaBIOTECH2021
A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners.
Article in aBIOTECH, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 16 citations in OpenAlex.
- TaSPX3 Enhances Wheat Resistance to Leaf Rust by Antagonising TaDi19-Mediated Repression of Pathogenesis-Related Genes.Plant biotechnology journal · 2026Article
- Lighting up yeast: overview of optogenetics in yeast and their applications to yeast biotechnology.FEMS yeast research · 2025Review
- Liquid-liquid phase separation of TZP promotes PPK-mediated phosphorylation of the phytochrome A photoreceptor.Nature plants · 2024Article
- Assessing the Function of CBF1 in Modulating the Interaction Between Phytochrome B and PIF4.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Reconstitution of phytochrome A-mediated light modulation of the ABA signaling pathways in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- SALT OVERLY SENSITIVE2 stabilizes phytochrome-interacting factors PIF4 and PIF5 to promote Arabidopsis shade avoidance.The Plant cell · 2023Article
- Novel and multifaceted regulations of photoperiodic flowering by phytochrome A in soybean.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- WRKY2 and WRKY10 regulate the circadian expression ofPlant communications · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Phytochromes are a family of photoreceptors in plants that perceive the red (R) and far-red (FR) components of their light environment. Phytochromes exist in vivo in two forms, the inactive Pr form and the active Pfr form, that are interconvertible by treatments with R or FR light. It is believed that phytochromes transduce light signals by interacting with their signaling partners. A GAL4-based light-switchable yeast two-hybrid (Y2H) system was developed two decades ago and has been successfully employed in many studies to determine phytochrome interactions with their signaling components. However, several pairs of interactions between phytochromes and their interactors, such as the phyA-COP1 and phyA-TZP interactions, were demonstrated by other assay systems but were not detected by this GAL4 Y2H system. Here, we report a modified LexA Y2H system, in which the LexA DNA-binding domain is fused to the C-terminus of a phytochrome protein. The conformational changes of phytochromes in response to R and FR light are achieved in yeast cells by exogenously supplying phycocyanobilin (PCB) extracted from Supplementary Information: The online version contains supplementary material available at 10.1007/s42994-021-00034-5.
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