ReviewFrontiers in bioinformatics2022
Multi-Dimensional Spectral Single Molecule Localization Microscopy.
Review in Frontiers in bioinformatics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Using DNA Origami to Study Nanoscale Organization of Plasma Membranes.Nano letters · 2026Article
- Membrane-associated effluxosomes coordinate multi-metal resistance in Mycobacterium tuberculosis.The EMBO journal · 2026Article
- Orthogonally Dispersed Spectroscopic Single-Molecule Localization Microscopy.Nanophotonics (Berlin, Germany) · 2026Article
- Affinity-guided labeling reveals P2X7 nanoscale membrane redistribution during BV2 microglial activation.eLife · 2026Article
- Parallel stopped-flow interrogation of diverse biological systems at the single-molecule scale.Nature methods · 2026Article
- DDHD2 provides a flux of saturated fatty acids for neuronal energy and function.Nature metabolism · 2025Article
- In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.Nature communications · 2025Article
- Hippo signaling regulates the nuclear behavior and DNA binding times of YAP and TEAD to control transcription.Science advances · 2025Article
- Spectral Hadamard microscopy with metasurface-based patterned illumination.Nanophotonics (Berlin, Germany) · 2025Article
- Monitoring Electrochemical Dynamics through Single-Molecule Imaging of hBN Surface Emitters in Organic Solvents.ACS nano · 2024Article
- Actin-driven nanotopography promotes stable integrin adhesion formation in developing tissue.Nature communications · 2024Article
- Real-time data processing in colorimetry camera-based single-molecule localization microscopy via CPU-GPU-FPGA heterogeneous computation.Biomedical optics express · 2024Article
- Synapsin 2a tetramerisation selectively controls the presynaptic nanoscale organisation of reserve synaptic vesicles.Nature communications · 2024Article
- Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis.Nature communications · 2024Article
- Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy.Chemical & biomedical imaging · 2023Review
- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Spectroscopic single-molecule localization microscopy: applications and prospective.Nano convergence · 2023Review
- Enabling Spectrally Resolved Single-Molecule Localization Microscopy at High Emitter Densities.Nano letters · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single molecule localization (SML) and tracking (SPT) techniques, such as (spt)PALM, (u/DNA)PAINT and quantum dot tracking, have given unprecedented insight into the nanoscale molecular organization and dynamics in living cells. They allow monitoring individual proteins with millisecond temporal resolution and high spatial resolution (<30 nm) by precisely localizing the point spread function (PSF) of individual emitters and tracking their position over time. While SPT methods have been extended to study the temporal dynamics and co-organization of multiple proteins, conventional experimental setups are restricted in the number of proteins they can probe simultaneously and usually have to tradeoff between the number of colors, the spatio-temporal resolution, and the field of view. Yet, localizing and tracking several proteins simultaneously at high spatial and temporal resolution within large field of views can provide important biological insights. By employing a dual-objective spectral imaging configuration compatible with live cell imaging combined with dedicated computation tools, we demonstrate simultaneous 3D single particle localization and tracking of multiple distinct species over large field of views to be feasible without compromising spatio-temporal resolution. The dispersive element introduced into the second optical path induces a spectrally dependent displacement, which we used to analytically separate up to five different fluorescent species of single emitters based on their emission spectra. We used commercially available microscope bodies aligned one on top of the other, offering biologists with a very ergonomic and flexible instrument covering a broad range of SMLM applications. Finally, we developed a powerful freely available software, called PALMTracer, which allows to quantitatively assess 3D + t + λ SMLM data. We illustrate the capacity of our approach by performing multi-color 3D DNA-PAINT of fixed samples, and demonstrate simultaneous tracking of multiple receptors in live fibroblast and neuron cultures.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.