Evidence map›Paper›PMID 36302792›Full record

ArticleScientific reports2022

Sarcomere dynamics revealed by a myofilament integrated FRET-based biosensor in live skeletal muscle fibers.

Ashley A Martin, Brian R Thompson, Jonathan P Davis, Hluechy Vang, Dongwoo Hahn, Joseph M Metzger

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Exercise metabolism and adaptation in skeletal muscle.Nature reviews. Molecular cell biology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ashley A MartinDepartment of Integrative Biology and Physiology, University of Minnesota Medical School, 6-125 Jackson Hall, 321 Church Street SE, Minneapolis, MN, 55455, USA.
Brian R ThompsonDepartment of Integrative Biology and Physiology, University of Minnesota Medical School, 6-125 Jackson Hall, 321 Church Street SE, Minneapolis, MN, 55455, USA.
Jonathan P DavisDepartment of Physiology and Cell Biology, Ohio State University, Columbus, OH, USA.
Hluechy VangDepartment of Integrative Biology and Physiology, University of Minnesota Medical School, 6-125 Jackson Hall, 321 Church Street SE, Minneapolis, MN, 55455, USA.
Dongwoo HahnDepartment of Integrative Biology and Physiology, University of Minnesota Medical School, 6-125 Jackson Hall, 321 Church Street SE, Minneapolis, MN, 55455, USA.
Joseph M MetzgerDepartment of Integrative Biology and Physiology, University of Minnesota Medical School, 6-125 Jackson Hall, 321 Church Street SE, Minneapolis, MN, 55455, USA. metzgerj@umn.edu.
University of Minnesota · USThe Ohio State University · US

Funding

Myofilaments as regulators of heart function in diseaseR01HL132874 · NHLBI · UNIVERSITY OF MINNESOTA · PI METZGER, JOSEPH MARK · 2017 to 2025
$3.7M
Skeletal muscle sarcomere function in health and diseaseR01AR079477 · NIAMS · UNIVERSITY OF MINNESOTA · PI JOSEPH Mark METZGER · 2022 to 2026
$2.6M
NHLBI NIH HHS R01 HL132874NIAMS NIH HHS R01 AR079477
6 · The paper itself

Abstract

The sarcomere is the functional unit of skeletal muscle, essential for proper contraction. Numerous acquired and inherited myopathies impact sarcomere function causing clinically significant disease. Mechanistic investigations of sarcomere activation have been challenging to undertake in the context of intact, live skeletal muscle fibers during real time physiological twitch contractions. Here, a skeletal muscle specific, intramolecular FRET-based biosensor was designed and engineered into fast skeletal muscle troponin C (TnC) to investigate the dynamics of sarcomere activation. In transgenic animals, the TnC biosensor incorporated into the skeletal muscle fiber sarcomeres by stoichiometric replacement of endogenous TnC and did not alter normal skeletal muscle contractile form or function. In intact single adult skeletal muscle fibers, real time twitch contractile data showed the TnC biosensor transient preceding the peak amplitude of contraction. Importantly, under physiological temperatures, inactivation of the TnC biosensor transient decayed significantly more slowly than the Ca

Indexed as

Biosensing TechniquesSarcomeresAnimalsCalciumFluorescence Resonance Energy TransferMuscle ContractionMuscle Fibers, SkeletalMuscle, SkeletalMyofibrilsTroponin CCalciumTroponin C

Identifiers

PMID36302792
PMCPMC9613882
OpenAlexW4307775768

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.