Evidence map›Paper›PMID 36302554›Full record

ArticleGenes & development2022

Single-molecule identification of the target RNAs of different RNA binding proteins simultaneously in cells.

Mathieu N Flamand, Ke Ke, Renee Tamming, Kate D Meyer

Open access · diamondAbstract read
In one paragraph

Article in Genes & development, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Comprehensive Profiling of NAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Single-cell mNature neuroscience · 2024
    Article
  17. Review
  18. Article
  19. Review
  20. Challenges to mapping and defining mRNA (New York, N.Y.) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Mathieu N Flamand *Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina 27710, USA.ORCID 0000-0002-2626-2303
Ke Ke *Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina 27710, USA.
Renee TammingDepartment of Biochemistry, Duke University School of Medicine, Durham, North Carolina 27710, USA.
Kate D MeyerDepartment of Biochemistry, Duke University School of Medicine, Durham, North Carolina 27710, USA.ORCID 0000-0001-7197-4054
Duke University · US

Funding

Epitranscriptomic Regulation of Synaptic Responses to Drugs of AbuseDP1DA046584 · NIDA · DUKE UNIVERSITY · PI MEYER, KATHRYN D · 2018 to 2022
$2.4M
Mechanistic Insights into m6A-Mediated Regulation of Brain DevelopmentR01MH118366 · NIMH · DUKE UNIVERSITY · PI MEYER, KATHRYN D · 2019 to 2023
$2.0M
CIHRNIDA NIH HHS DP1 DA046584NIMH NIH HHS R01 MH118366
6 · The paper itself

Abstract

RNA-binding proteins (RBPs) regulate nearly every aspect of mRNA processing and are important regulators of gene expression in cells. However, current methods for transcriptome-wide identification of RBP targets are limited, since they examine only a single RBP at a time and do not provide information on the individual RNA molecules that are bound by a given RBP. Here, we overcome these limitations by developing TRIBE-STAMP, an approach for single-molecule detection of the target RNAs of two RNA binding proteins simultaneously in cells. We applied TRIBE-STAMP to the cytoplasmic m

Indexed as

RNARNA-Binding ProteinsRNA, MessengerRNA Processing, Post-TranscriptionalTranscriptomeRNARNA-Binding ProteinsRNA, Messengerm6Am6A readersRNA binding proteinsSTAMPTRIBETRIBE-STAMPYTHDF

Identifiers

PMID36302554
PMCPMC9732904
OpenAlexW4307502246

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.