Evidence map›Paper›PMID 36301341›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2022

C/EBPβ enhances immunosuppression activity of myeloid-derived suppressor cells by a P300-mediated acetylation modification.

Wenxin Wang, Yuxuan Chen, Rongrong Du, Xueli Xia, Yue Zhang, Hongye Guo, Jie Ma, Jie Tian, Shengjun Wang

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Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Wenxin WangDepartment of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Dianli Road No 8, Zhenjiang, 212002, China.
Yuxuan ChenDepartment of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Rongrong DuDepartment of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Xueli XiaDepartment of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Yue ZhangDepartment of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Dianli Road No 8, Zhenjiang, 212002, China.
Hongye GuoDepartment of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Jie MaDepartment of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Jie TianDepartment of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Shengjun WangDepartment of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Dianli Road No 8, Zhenjiang, 212002, China. sjwjs@ujs.edu.cn.

Funding

Research Project of the Jiangsu Commission of Health K2019019
6 · The paper itself

Abstract

objectiveMyeloid-derived suppressor cells (MDSCs) are a major immunosuppressive population in the tumor microenvironment,inhibiting anti-tumor immune response and exerting pro-tumorigenic effect. CCAAT/enhancer-binding protein beta (C/EBPβ), a key transcription factor indispensable for myelopoiesis, plays a fundamental role in regulating expansion and activation of MDSCs. Lysine acetylation can regulate functions of transcription factors. However, the role of C/EBPβ acetylation modification in MDSCs has not been reported. MATERIALS AND

methodsMDSCs derived from the spleens of tumor-bearing mice (TB-SP-MDSCs) were isolated by immunomagnetic beads. Bone marrow derived MDSCs were induced by IL-6 and GM-CSF. Western-blot was used to detect the expression of P300 and co-immunoprecipitation (CO-IP) was used to detect the C/EBPβ acetylation in MDSCs. Inhibitor C646 was used to specificly inhibit P300 activity.

resultsIn this study, we found that C/EBPβ was acetylated by acetyltransferase P300 in MDSCs. A P300-mediated C/EBPβ acetylation enhanced C/EBPβ transactivation activity on arginase 1 (Arg-1) gene promoter. Inhibition of P300 activity downregulated the inhibitory effects of MDSCs in vitro and attenuated pro-tumorigenic effects of MDSCs in vivo. Additionally, IL-6 from tumor microenvironment could upregulate the expression of P300 and enhance C/EBPβ acetylation in MDSCs.

conclusionIn general, a P300-mediated C/EBPβ acetylation enhanced C/EBPβ transactivation activity on Arg-1 promoter, thus promoting immunosuppressive function of MDSCs. In view of the critical role of P300 in regulating MDSCs, P300 might be a potential target of anti-tumor immunotherapy.

Indexed as

Myeloid-Derived Suppressor CellsNeoplasmsAcetylationAnimalsCCAAT-Enhancer-Binding Protein-betaImmune ToleranceImmunosuppression TherapyInterleukin-6MiceTumor MicroenvironmentCCAAT-Enhancer-Binding Protein-betaInterleukin-6AcetylationC/EBPβImmunosuppressionMyeloid-derived suppressor cellsP300

Identifiers

PMID36301341

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.