Fast and Non-Destructive Quail Egg Freshness Assessment Using a Thermal Camera and Deep Learning-Based Air Cell Detection Algorithms for the Revalidation of the Expiration Date of Eggs.

Freshness is one of the most important parameters for assessing the quality of avian eggs. Available techniques to estimate the degradation of albumen and enlargement of the air cell are either destructive or not suitable for high-throughput applications. The aim of this research was to introduce a new approach to evaluate the air cell of quail eggs for freshness assessment as a fast, noninvasive, and nondestructive method. A new methodology was proposed by using a thermal microcamera and deep learning object detection algorithms. To evaluate the new method, we stored 174 quail eggs and collected thermal images 30, 50, and 60 days after the labeled expiration date. These data, 522 in total, were expanded to 3610 by image augmentation techniques and then split into training and validation samples to produce models of the deep learning algorithms, referred to as "You Only Look Once" version 4 and 5 (YOLOv4 and YOLOv5) and EfficientDet. We tested the models in a new dataset composed of 60 eggs that were kept for 15 days after the labeled expiration label date. The validation of our methodology was performed by measuring the air cell area highlighted in the thermal images at the pixel level; thus, we compared the difference in the weight of eggs between the first day of storage and after 10 days under accelerated aging conditions. The statistical significance showed that the two variables (air cell and weight) were negatively correlated (R