Evidence map›Paper›PMID 36296629›Full record

ArticleMolecules (Basel, Switzerland)2022

Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies.

Chun-Ho Charlie Ma, Liejun Li, Shuheng Cai, Pei Lin, Wing-Ki Kristy Lam, Tsz-Him Ronald Lee, Tsz-Kin Ryan Kwok, Longxu Xie, Tit-Sang Tom Kun, Ben-Zhong Tang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chun-Ho Charlie MaDepartment of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Liejun LiGuangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China.
Shuheng CaiGuangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China.
Pei LinGuangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China.
Wing-Ki Kristy LamDepartment of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.ORCID 0000-0002-5745-8667
Tsz-Him Ronald LeeDepartment of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Tsz-Kin Ryan KwokDepartment of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Longxu XieGuangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China.
Tit-Sang Tom KunGuangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China.
Ben-Zhong TangDepartment of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.

Funding

The Innovation and Technology Commission ITS/301/18FX and ITC-CNERC14SC01
6 · The paper itself

Abstract

Genetic mutations can cause life-threatening diseases such as cancers and sickle cell anemia. Gene detection is thus of importance for disease-risk prediction or early diagnosis and treatment. Apart from genetic defects, gene detection techniques can also be applied to gene-related diseases with high risk to human health such as human papillomavirus (HPV) infection. HPV infection has been strongly linked to cervical cancer. To achieve a high-throughput HPV gene detection platform, the flow-through hybridization system appears to be one of the commercialized diagnostic techniques for this purpose. The flow-through hybridization technique is based on a vacuum-guided flow of DNA fragments which is continuously directed toward the oligoprobes that are immobilized on the testing membrane. However, the conventional colorimetric method and signal read-out approach suffers a problem of low sensitivity. On the contrary, fluorescence approaches allow more sensitive detection and broad sensing ranges. In this work, a fluorescent dye HCAP, which possesses aggregation-induced emission (AIE) properties and is responsive to alkaline phosphatase, was developed and applied to the flow-through hybridization platform to achieve HPV genome diagnosis of clinical samples. Also, an automatic membrane reader was constructed based on the AIE-based diagnosis platform which can identify the diagnostic result of patient DNA with a total concordance rate of 100% in the clinical trial.

Indexed as

Papillomavirus InfectionsUterine Cervical NeoplasmsAlkaline PhosphataseCervix UteriDNA, ViralFemaleFluorescent DyesGenotypeHumansPapillomaviridaeAlkaline PhosphataseDNA, ViralFluorescent Dyesaggregation-induced emissionalkaline phosphataseflow-through hybridizationgenetic diseaseshuman immunodeficiency virusmembrane reader

Identifiers

PMID36296629
PMCPMC9609701

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.