Evidence map›Paper›PMID 36294565›Full record

ArticleJournal of fungi (Basel, Switzerland)2022

A Simple and Efficient CRISPR/Cas9 System Using a Ribonucleoprotein Method for

Jianyu Liu, Haiyang Cui, Ruijuan Wang, Zhen Xu, Hailong Yu, Chunyan Song, Huan Lu, Qiaozhen Li, Danrun Xing, Qi Tan and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Development of a CRISPR/Cas9 Genome Editing System in DikaryoticJournal of fungi (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Article
  5. Towards engineering agaricomycete fungi for terpenoid production.Journal of industrial microbiology & biotechnology · 2024
    Review
  6. Effect of a Mating Type Gene Editing inJournal of fungi (Basel, Switzerland) · 2024
    Article
  7. Review
  8. Review
  9. Biotechnology of Edible Fungi.Journal of fungi (Basel, Switzerland) · 2023
    Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Jianyu LiuNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Haiyang CuiCollege of Marine Resources and Environment, Hebei Normal University of Science & Technology, Qinghuangdao 066004, China.ORCID 0000-0003-3706-9338
Ruijuan WangNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Zhen XuNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Hailong YuNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Chunyan SongNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Huan LuNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Qiaozhen LiNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Danrun XingCollege of Marine Resources and Environment, Hebei Normal University of Science & Technology, Qinghuangdao 066004, China.
Qi TanNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Weiming SunCollege of Marine Resources and Environment, Hebei Normal University of Science & Technology, Qinghuangdao 066004, China.
Gen ZouNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.ORCID 0000-0001-5574-1824
Xiaodong ShangNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Shanghai Academy of Agricultural Sciences · CNHebei Normal University of Science and Technology · CN

Funding

Shanghai Agriculture Applied Technology Development Program, China Grant No.G20200103
6 · The paper itself

Abstract

CRISPR/Cas9 systems were established in some edible fungi based on in vivo expressed Cas9 and guide RNA. Compared with those systems, the in vitro assembled Cas9 and sgRNA ribonucleoprotein complexes (RNPs) have more advantages, but only a few examples were reported, and the editing efficiency is relatively low. In this study, we developed and optimized a CRISPR/Cas9 genome-editing method based on in vitro assembled ribonucleoprotein complexes in the mushroom

Indexed as

CRISPR/Cas9Flammulina filiformisgenomic editingpyrGRNPs

Identifiers

PMID36294565
PMCPMC9604558
OpenAlexW4297201610

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.