Evidence map›Paper›PMID 36293301›Full record

ArticleInternational journal of molecular sciences2022

In Vitro Assessment of Poly-N-Vinylpyrrolidone/Acrylic Acid Nanoparticles Biocompatibility in a Microvascular Endothelium Model.

Aikaterini Berdiaki, Andrey N Kuskov, Pavel P Kulikov, Lydia-Nefeli Thrapsanioti, Eirini-Maria Giatagana, Polychronis Stivaktakis, Mikhail I Shtilman, Aristidis Tsatsakis, Dragana Nikitovic

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Aikaterini BerdiakiLaboratory of Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece.
Andrey N KuskovDepartment of Technology of Chemical Pharmaceutical and Cosmetic Substances, D. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia.ORCID 0000-0001-8140-2754
Pavel P KulikovCentre for Strategic Planning of FMBA of Russia, 119121 Moscow, Russia.ORCID 0000-0001-5221-7613
Lydia-Nefeli ThrapsaniotiLaboratory of Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece.
Eirini-Maria GiataganaLaboratory of Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece.
Polychronis StivaktakisLaboratory of Toxicology, School of Medicine, University of Crete, 71003 Heraklion, Greece.
Mikhail I ShtilmanDepartment of Biomaterials, Mendeleev University of Chemical Technology of Russia, Miusskaya sqr. 9, 125047 Moscow, Russia.
Aristidis TsatsakisLaboratory of Toxicology, School of Medicine, University of Crete, 71003 Heraklion, Greece.ORCID 0000-0003-3824-2462
Dragana NikitovicLaboratory of Histology-Embryology, School of Medicine, University of Crete, 71003 Heraklion, Greece.ORCID 0000-0003-3882-7399
University of Crete · GRD. Mendeleyev University of Chemical Technology of Russia · RUCenter for Strategic Research · RU

Funding

Research Committee of the University of Crete (ELKE) 10985
6 · The paper itself

Abstract

An amphiphilic copolymer of N-vinyl-2-pyrrolidone and acrylic acid-namely, p(VP-AA)-OD6000 (p(VP-AA))-was synthesized to prepare p(VP-AA) nanoparticles (NPs). Furthermore, the copolymer was linked with CFSE, and the so-prepared nanoparticles were loaded with the DiI dye to form D nanoparticles (DNPs). In this study, as demonstrated by immunofluorescence microscopy, immunofluorescence, and confocal microscopy, DNPs were readily taken up by human microvascular endothelial cells (HMEC-1) cells in a concentration-dependent manner. Upon uptake, both the CFSE dye (green stain) and the DiI dye (red stain) were localized to the cytoplasm of treated cells. Treatment with p(VP-AA) did not affect the viability of normal and challenged with LPS, HMEC-1 cells at 0.010 mg/mL and induced a dose-dependent decrease of these cells' viability at the higher concentrations of 0.033 and 0.066 mg/mL (

Indexed as

E-SelectinNanoparticlesAcrylatesEndothelial CellsEndotheliumFluoresceinsHumansIntercellular Adhesion Molecule-1LipopolysaccharidesPolymersPyrrolidinonesSuccinimides5-(6)-carboxyfluorescein diacetate succinimidyl esterAcrylatesacrylic acidE-SelectinFluoresceinsIntercellular Adhesion Molecule-1LipopolysaccharidesN-vinyl-2-pyrrolidinonePolymersPyrrolidinonesSuccinimidesacrylic acidamphiphilic polymerassessmentendothelial cellsfluorescent probesimmunological activationnanoparticlepoly-N-vinylpyrrolidoneviability

Identifiers

PMID36293301
PMCPMC9604021
OpenAlexW4306742147

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.