ArticleInternational journal of molecular sciences2022
Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Biomimetic Fibrin Matrix Modulates Early Human Follicular Growth Dynamics in a Bioengineered Artificial Ovary Derived from Cryopreserved Tissue: In Vitro Morphometric Assessment.International journal of molecular sciences · 2026Article
- Dynamic in vitro 3D culture of cryopreserved human ovarian tissue: transcriptomic analysis by RNA sequencing.Journal of ovarian research · 2026Article
- Fertility under fire: how chemotherapy harms the ovaries and the science fighting back?Journal of ovarian research · 2026Review
- A diagnosis and treatment algorithm for adnexal masses in female children and adolescents.World journal of pediatric surgery · 2026Article
- Wharton's Jelly Hydrogel: An Innovative Artificial Ovary for Xenotransplantation of Isolated Human Ovarian Follicles.Biology · 2025Article
- FGA, a new target of histone acetylation, inhibits apoptosis of granulosa cells in follicles.Biological research · 2025Article
- Construction and Bioengineering of Human Bioprosthetic Ovaries from Cryopreserved Ovarian Tissue.International journal of molecular sciences · 2025Review
- Fertility Preservation in Female Children and Adolescent Cancer Patients.Children (Basel, Switzerland) · 2025Review
- Role of Ovarian Stem Cells in Postnatal Oogenesis and Implications in Fertility Preservation.Advances in experimental medicine and biology · 2025Review
- Research progress on the prevention and treatment of chemotherapy-induced ovarian damage.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2024Review
- Assessment of molecular and morphological dynamics during long-timeFrontiers in endocrinology · 2024Article
- Innovative Strategies for Fertility Preservation in Female Cancer Survivors: New Hope from Artificial Ovary Construction and Stem Cell-Derived Neo-Folliculogenesis.Healthcare (Basel, Switzerland) · 2023Review
- RNA Transcripts in Human Ovarian Cells: Two-Time Cryopreservation Does Not Affect Developmental Potential.International journal of molecular sciences · 2023Article
- Morphological and Chemical Investigation of Ovarian Structures in a Bovine Model by Contrast-Enhanced X-ray Imaging and Microscopy.International journal of molecular sciences · 2023Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe in vitro culture of primordial follicles is the only available option for preserving fertility in prepubertal girls with malignant tumors. The cultivation of primordial follicles in scaffolds as artificial ovaries is a promising approach for this.
methodsDissociated follicles were placed into an artificial ovarian scaffold composed of fibrinogen and thrombin. The follicles were cultured in a dish dedicated to live cell imaging and observed for growth using immunofluorescence and development via optical microscopy. The morphology of the follicles in the scaffold was three-dimensionally reconstructed using the Imaris software. Growth and development were also quantified.
resultsThe morphology of artificial ovaries began to degrade over time. Within approximately 7 days, primordial follicles were activated and grew into secondary follicles. A comparison of optical and confocal microscopy results revealed the superior detection of live cells using confocal microscopy. The three-dimensional reconstruction of the confocal microscopy data enabled the automatic enumeration and evaluation of the overall morphology of many follicles.
conclusionsThe novel artificial ovary-enabled primordial follicles to enter the growth cycle after activation and grow into secondary follicles. The use of a fibrin scaffold as a carrier preserves the developmental potential of primordial germ cells and is a potentially effective method for preserving fertility in prepubertal girls.
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