Evidence map›Paper›PMID 36291255›Full record

ArticleBrain sciences2022

Exosomes derived from M2 Macrophages Improve Angiogenesis and Functional Recovery after Spinal Cord Injury through HIF-1α/VEGF Axis.

Jiang-Hu Huang, Hang He, Yong-Neng Chen, Zhen Liu, Manini Daudi Romani, Zhao-Yi Xu, Yang Xu, Fei-Yue Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 2 pooled it
3.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 2 syntheses or guidelines pooled it, 44 citations in OpenAlex.

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  5. Extracellular vesicle-based therapeutic strategies for spinal cord injury.Extracellular vesicles and circulating nucleic acids · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Jiang-Hu HuangDepartment of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, China.
Hang HeDepartment of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, China.
Yong-Neng ChenDepartment of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, China.
Zhen LiuDepartment of Social Economy and Business Administration, Woosuk University, Wanju-gun 55338, Korea.
Manini Daudi RomaniDepartment of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhao-Yi XuDepartment of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, China.
Yang XuDepartment of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, China.
Fei-Yue LinDepartment of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, China.ORCID 0000-0002-9184-0229
Fujian Medical University · CNCentral South University · CNWoosuk University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes are nano-sized vesicles that contain a variety of mRNAs, miRNAs, and proteins. They are capable of being released by a variety of cells and are essential for cell-cell communication. The exosomes produced by cells have shown protective benefits against spinal cord damage (SCI). Recently, it was discovered that M2 macrophages aid in the angiogenesis of numerous illnesses. However, the functional role of M2 macrophage-derived exosomes on SCI is unclear. Here, we investigate the pro-angiogenesis of M2 macrophage-derived exosomes on SCI. We founded that M2 macrophage exosomes alleviated tissue damage and enhanced functional recovery post-SCI. We discovered that M2 macrophage exosome administration increased angiogenesis after SCI in vivo using immunohistochemistry, immunofluorescence labeling, and Western blot analysis. Additionally, the expression of the pro-angiogenesis factors, HIF-1α and VEGF, were enhanced with the treatment of the M2 macrophage exosomes. Furthermore, we found that M2 macrophage exosomes enhanced neurogenesis after SCI in vivo. In vitro, we found that M2 macrophage exosomes can be taken up by the brain endothelial cell line (bEnd.3) and that they enhanced the tube formation, migration, and proliferation of bEnd.3 cells. Furthermore, by using special siRNA to inhibit HIF-1α expression, we observed that the expression of VEGF decreased, and the tube formation, migration, and proliferation of bEnd.3 cells were attenuated with the treatment of HIF-1α-siRNA. In conclusion, our findings reveal that M2 macrophage exosomes improve neurological functional recovery and angiogenesis post-SCI, and this process is partially associated with the activation of the HIF-1/VEGF signaling pathway.

Indexed as

angiogenesisfunctional recoveryHIF-1αM2 macrophage-derived exosomesspinal cord injury

Identifiers

PMID36291255
PMCPMC9599527
OpenAlexW4297988860

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.