ArticleAnimals : an open access journal from MDPI2022
Effect of Dimethylacetamide Concentration on Motility, Quality, Antioxidant Biomarkers, Anti-Freeze Gene Expression, and Fertilizing Ability of Frozen/Thawed Rooster Sperm.
Article in Animals : an open access journal from MDPI, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Evaluation of sperm kinematics, physical integrity and malondialdehyde levels as indicators of sperm freezability in Pradu Hang Dam chicken.Veterinary research communications · 2026Article
- Cryopreservation of Human Spermatozoa Using Penetrating Cryoprotectant-Free Vitrification Medium Exhibits an Altered Proteomic Profile Compared to the Conventional Rapid Freezing Method.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Effects of Kaempferol Supplementation on the Cryopreservation Quality of Semen from Yuansheng Aite Dairy Rams.Antioxidants (Basel, Switzerland) · 2026Article
- Integrating morphometrics and seminal plasma metabolomics to predict fertility in Yoruba Ecotype × Sussex crossbred cocks.Veterinary world · 2025Article
- Correlation Analysis of Sperm Cryopreservation Quality with Serum Testosterone and Sperm gDNA Methylation Levels in Xiaoshan Chickens.Animals : an open access journal from MDPI · 2025Article
- Loss ofInternational journal of molecular sciences · 2025Article
- Effect of semen dilution rate and dimethyl acetamide levels on post-thaw motility and fertility parameters of rooster sperm.PloS one · 2025Article
- Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function.Scientific reports · 2024Article
- The In Vitro Evaluation of Rooster Semen Pellets Frozen with Dimethylacetamide.Animals : an open access journal from MDPI · 2023Article
- Supplementing semen extenders with a combination of phosphorus and vitamin B12 Improves post-thawed cryopreserved rooster semen quality.Frontiers in veterinary science · 2023Article
- Current Status and Advances in Semen Preservation.Animals : an open access journal from MDPI · 2022Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 5 countries.
Funding
Abstract
Sperm cryopreservation is of great importance for the poultry industry but still needs to be optimized. The high susceptibility of poultry sperm to cryodamage leads to low fertility rates after cryopreservation. Therefore, the present study aimed at evaluating the effect of including a cryoprotectant, dimethylacetamide (DMA), in the chicken semen freezing extenders at a final concentration of 3%, 6%, or 9% on the post-thawed sperm motility, quality, antioxidant biomarkers, anti-freeze gene expression, and fertilizing ability. Results showed that the total motile sperm, progressivity, and viability were quadratically increased (p < 0.05) in the 6% DMA group. The antioxidant enzyme activity and lipid peroxidation were negatively (p < 0.05) affected by the increase in DMA concentration. Furthermore, some anti-freeze-associated genes such as heat shock protein 70 (HSP70) and ras homolog family member A (RHOA) were linearly and quadratically down-regulated (p < 0.05) with the high concentration of DMA. Finally, the fertility and hatchability rates did not indicate statistical differences between DMA groups. It can be concluded that using the low concentration of 3−6% DMA in the freezing semen extender is preferable to obtain acceptable results in the post-thawed sperm quality and fertility.
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