Transporter-dependent uptake and metabolism of myocardial interstitial serotonin in the rat heart.

Takashi Sonobe, Tsuyoshi Akiyama, James T Pearson

Open access · goldAbstract read

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Article in The journal of physiological sciences : JPS, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 74% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Takashi SonobeDepartment of Cardiac Physiology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan. tsonobe@ncvc.go.jp.ORCID http://orcid.org/0000-0003-3589-0405

Tsuyoshi AkiyamaDepartment of Cardiac Physiology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan.

James T PearsonDepartment of Cardiac Physiology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan.

National Cerebral and Cardiovascular Center · JPAustralian Regenerative Medicine Institute · AU

Funding

Japan Society for the Promotion of Science JP17K11604Japan Society for the Promotion of Science JP18K10882Japan Society for the Promotion of Science JP21K11358Japan Society for the Promotion of Science JP22K09182

6 · The paper itself

Abstract

To investigate the roles of the serotonin (5-HT) transporter (SERT) and plasma membrane monoamine transporter (PMAT) in 5-HT uptake and its metabolism in the heart, we monitored myocardial interstitial levels of 5-HT and 5-HIAA, a metabolite of 5-HT by monoamine oxidase (MAO), in anesthetized rats using a microdialysis technique. Fluoxetine (SERT inhibitor), decynium-22 (PMAT inhibitor), or their mixture was locally administered by reverse-microdialysis for 60 min. Subsequently, pargyline (MAO inhibitor) was co-administered. Fluoxetine rapidly increased dialysate 5-HT concentration, while decynium-22 gradually increased it. The mixture induced a larger increase in dialysate 5-HT concentration compared to fluoxetine or decynium-22 alone. Fluoxetine increased dialysate 5-HIAA concentration, and this increase was abolished by pargyline. Decynium-22 and the mixture did not change dialysate 5-HIAA concentration, which were not affected by pargyline. Both SERT and PMAT regulate myocardial interstitial 5-HT levels by its uptake; however, 5-HT uptake via PMAT leads to 5-HT metabolism by MAO.

Indexed as

Monoamine Oxidase InhibitorsSerotoninAnimalsDialysis SolutionsFluoxetineHeartHydroxyindoleacetic AcidMembrane Transport ProteinsMonoamine OxidasePargylineRatsDialysis SolutionsFluoxetineHydroxyindoleacetic AcidMembrane Transport ProteinsMonoamine OxidaseMonoamine Oxidase InhibitorsPargylineSerotonin5-HIAA5-HTCardiac microdialysisMonoamine oxidasePlasma membrane monoamine transporterSerotonin transporter

Identifiers

PMID36289481

DOI10.1186/s12576-022-00852-2

PMCPMC10717708

OpenAlexW4307498805

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.