ArticleMethods and protocols2022
Sequential Isolation of Microglia and Astrocytes from Young and Aged Adult Mouse Brains for Downstream Transcriptomic Analysis.
Article in Methods and protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Peripheral macrophages and T-cells accumulate in the degenerating optic tract after repetitive head impact.Brain, behavior, and immunity · 2026Article
- Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system.STAR protocols · 2026Article
- Article
- Loss of insulin signaling in microglia impairs cellular uptake of Aβ and neuroinflammatory response exacerbating AD-like neuropathology.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Microglia isolation from aging mice for cell culture: A beginner's guide.Frontiers in cellular neuroscience · 2023Article
- Traumatic brain injury induces an adaptive immune response in the meningeal transcriptome that is amplified by aging.Frontiers in neuroscience · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In aging, the brain is more vulnerable to injury and neurodegenerative disease, but the mechanisms responsible are largely unknown. Evidence now suggests that neuroinflammation, mediated by resident brain astrocyte and microglia populations, are key players in the generation of inflammatory responses and may influence both age related processes and the initiation/progression of neurodegeneration. Consequently, targeting these cell types individually and collectively may aid in the development of novel disease-modifying therapies. We have optimized and characterized a protocol for the effective sequential isolation of both microglia and astrocytes from the adult mouse brain in young and aged mice. We demonstrate a technique for the sequential isolation of these immune cells by using magnetic beads technology, optimized to increase yield and limit potential artifacts in downstream transcriptomic applications, including RNA-sequencing pipelines. This technique is versatile, cost-effective, and reliable for the study of responses within the same biological context, simultaneously being advantageous in reducing mice numbers required to assess cellular responses in normal and age-related pathological conditions.
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Registered trials
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