Integration of the Exogenous Tuning of Thraustochytrid Fermentation and Sulfur Polymerization of Single-Cell Oil for Developing Plant-like Oils.

Adarsha Gupta, Max J H Worthington, Justin M Chalker, Munish Puri

Open access · goldAbstract read

In one paragraph

Article in Marine drugs, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.3field-weighted citation impact, top 47% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Adarsha GuptaMedical Biotechnology, Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Bedford Park, Adelaide, SA 5042, Australia.ORCID 0000-0003-0873-7464

Max J H WorthingtonKey Centre for Polymers and Colloids, School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.

Justin M ChalkerInstitute for Nanoscale Science and Technology, College of Science and Engineering, Flinders University, Bedford Park, Adelaide, SA 5042, Australia.

Munish PuriMedical Biotechnology, Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Bedford Park, Adelaide, SA 5042, Australia.ORCID 0000-0003-2469-3326

Flinders University · AUThe University of Sydney · AU

Funding

Australian Research Council (DP200100090Flinders University Industry partnership seed grants IPSG 2020

6 · The paper itself

Abstract

In this study, we have demonstrated a bioprocessing approach encompassing the exogenous addition of low-molecular-weight compounds to tune the fatty acid (FA) profile in a novel thraustochytrid strain to produce desirable FAs. Maximum lipid recovery (38%, dry wt. biomass) was obtained at 1% Tween 80 and 0.25 mg/L of Vitamin B12. The transesterified lipid showed palmitic acid (C16, 35.7% TFA), stearic acid (C18, 2.1% TFA), and oleic acid (C18:1, 18.7% TFA) as the main components of total FAs, which are mainly present in plant oils. Strikingly, D-limonene addition in the fermentation medium repressed the production of polyunsaturated fatty acid (PUFAs). Sulfur-polymerization-guided lipid separation revealed the presence of saturated (SFAs, 53% TFA) and monounsaturated fatty acids (MUFAs, 46.6% TFA) in thraustochytrid oil that mimics plant-oil-like FA profiles. This work is industrially valuable and advocates the use of sulfur polymerization for preparation of plant-like oils through tuneable thraustochytrid lipids.

Indexed as

Fatty AcidsPolysorbatesFatty Acids, MonounsaturatedFatty Acids, UnsaturatedFermentationLimoneneOleic AcidPalmitic AcidsPlant OilsPolymerizationStearic AcidsSulfurVitamin B 12Fatty AcidsFatty Acids, MonounsaturatedFatty Acids, UnsaturatedLimoneneOleic AcidPalmitic AcidsPlant OilsPolysorbatesStearic AcidsSulfurVitamin B 12cell factoryfermentationomega-3 fatty acidplant oilspolyunsaturated fatty acidssulfur polymerization

Identifiers

PMID36286478

DOI10.3390/md20100655

PMCPMC9604933

OpenAlexW4307273063

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.