Evidence map›Paper›PMID 36286160›Full record

ArticleGels (Basel, Switzerland)2022

Osmanthus Fragrans Loaded NIPAAM Hydrogel Promotes Osteogenic Differentiation of MC3T3-E1.

Bin Huang, Mengyao Zhao, Mingzhe Yang, Lu Rao, Chizhou Wu, Yuzhu Hu, Huangqin Chen, Yuesheng Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gels (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Bin HuangDepartment of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China.
Mengyao ZhaoDepartment of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China.
Mingzhe YangDepartment of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China.
Lu RaoHubei Key Laboratory of Radiation Chemistry and Functional Materials, Non-Power Nuclear Technology Collaborative Innovation Center, Hubei University of Science and Technology, Xianning 437100, China.
Chizhou WuDepartment of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China.
Yuzhu HuDepartment of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China.
Huangqin ChenDepartment of Stomatology, School of Stomatology and Ophthalmology, Xianing Medical College, Hubei University of Science and Technology, Xianning 437100, China.
Yuesheng LiHubei Key Laboratory of Radiation Chemistry and Functional Materials, Non-Power Nuclear Technology Collaborative Innovation Center, Hubei University of Science and Technology, Xianning 437100, China.
Hubei University of Science and Technology · CN

Funding

Hubei Provincial Colleges and Universities Outstanding Young and Middle-aged Technological Innovation Team Project T2020022PhD start-up fund of Hubei University of Science and Technology 2018-20XB012Provincial College Students Innovation Training Program S202210927030, S202210927031Special fund of School of Stomatology and Ophthalmology of Hubei University of Science and Technology 2020XZ32, 2020WG01The Science Development Foundation of Hubei University of Science & Technology 2020TD01, 2021ZX01, 2022FH09Xianning City Key Program of Science & Technology 2021GXYF021
6 · The paper itself

Abstract

There is an urgent need to find long-acting, natural osteogenesis-promoting drug systems. In this study, first the potential targets and mechanism of osmanthus fragrans (O. fragrans) extract in regulating osteogenic differentiation based on autophagy were analyzed by network pharmacology and molecular docking. Then, osmanthus fragrans was extracted using the ethanol reflux method and an osmanthus fragrans extract loaded Poly N-isopropylacrylamide (OF/NIPAAM) hydrogel was prepared by electron beam radiation. The chemical components of the osmanthus fragrans extract and the microstructure of OF/NIPAAM hydrogels were characterized by ultraviolet-visible spectrophotometry (UV-Vis) and X-ray diffraction (XRD), respectively. Mouse embryonic osteoblast precursor cells MC3T3-E1 were cultured with different concentrations of OF/NIPAAM hydrogel to discover cell proliferation activity by CCK-8 assay. Alkaline phosphatase (ALP) staining and alizarin red staining were used to observe the differentiation and calcification. Through experimental exploration, we found that a total of 11 targets were predicted, which are TP53, CASP3, SIRT1, etc., and osmanthus fragrans had good binding activity to TP53. In vitro, except for proliferation promotion, OF/NIPAAM hydrogel enhanced ALP activity and formation of mineralized nodules of MC3T3-E1 cells at a concentration equal to or less than 62.5 μg/mL (p < 0.05). The addition of autophagy inhibitor 3-methyladenine (3-MA) reduced ALP activity and mineralized nodule formation.

Indexed as

electron beam radiationhydrogelmolecular dockingnetwork pharmacologyosmanthus fragrans-loaded NIPAAMosteogenic differentiation

Identifiers

PMID36286160
PMCPMC9601630
OpenAlexW4306377278

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.