Evidence map›Paper›PMID 36284311›Full record

ArticleGenome biology2022

CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice.

Masayuki Tanaka, Keiko Yokoyama, Hideki Hayashi, Sanae Isaki, Kanae Kitatani, Ting Wang, Hisako Kawata, Hideyuki Matsuzawa, Channabasavaiah B Gurumurthy, Hiromi Miura and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Masayuki Tanaka *Support Center for Medical Research and Education, Tokai University, Isehara, Kanagawa, 259-1193, Japan. matanaka@tokai-u.jp.
Keiko Yokoyama *Support Center for Medical Research and Education, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Hideki HayashiSupport Center for Medical Research and Education, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Sanae IsakiSupport Center for Medical Research and Education, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Kanae KitataniSupport Center for Medical Research and Education, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Ting WangSupport Center for Medical Research and Education, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Hisako KawataSupport Center for Medical Research and Education, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Hideyuki MatsuzawaSupport Center for Medical Research and Education, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Channabasavaiah B GurumurthyMouse Genome Engineering Core Facility, University of Nebraska Medical Center, Omaha, NE, USA.
Hiromi MiuraDivision of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan.
Masato OhtsukaDivision of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Isehara, Kanagawa, 259-1193, Japan. masato@is.icc.u-tokai.ac.jp.ORCID 0000-0002-6952-4238

Funding

Development of Modular CRISPR Genome Editing Technologies and ToolsR35HG010719 · NHGRI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GURUMURTHY, CHANNABASAVAIAH · 2019 to 2023
$2.2M
Engineering Long ssDNA for Genome Editing ApplicationsR21GM129559 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GURUMURTHY, CHANNABASAVAIAH, WHITEHEAD, TIMOTHY ANDREW · 2019 to 2020
$414k
NHGRI NIH HHS R35 HG010719NHGRI NIH HHS R35HG010719NIGMS NIH HHS R21 GM129559NIGMS NIH HHS R21GM129559
6 · The paper itself

Abstract

CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily incorporated as an additional step to genotype founder animals for further breeding.

Indexed as

Clustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsAnimalsDNAGene EditingGene Knock-In TechniquesGenomeMiceDNACIRCLE-seqCRISPRCRISPR-KRISPRDonor DNAIntronic regionKnock-inOff targetRandom insertionRepeat element

Identifiers

PMID36284311
PMCPMC9594901

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.