Evidence map›Paper›PMID 36284269›Full record

ArticleCellular & molecular biology letters2022

Circulating small extracellular vesicle-encapsulated SEMA5A-IT1 attenuates myocardial ischemia-reperfusion injury after cardiac surgery with cardiopulmonary bypass.

Ting Wu, Guoning Shi, Zhenhua Ji, Shu Wang, Lizhu Geng, Zhigang Guo

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular & molecular biology letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ting WuDepartment of Cardiopulmonary Bypass, Clinical College of Chest, Tianjin Medical University, Tianjin, China.
Guoning ShiDepartment of Cardiopulmonary Bypass, Chest Hospital, Tianjin University, Tianjin, China.
Zhenhua JiDepartment of Cardiopulmonary Bypass, Chest Hospital, Tianjin University, Tianjin, China.
Shu WangDepartment of Cardiopulmonary Bypass, Chest Hospital, Tianjin University, Tianjin, China.
Lizhu GengDepartment of Cardiopulmonary Bypass, Chest Hospital, Tianjin University, Tianjin, China.
Zhigang GuoDepartment of Cardiovascular Surgery, Chest Hospital, Tianjin University, No. 261, Taierzhuang South Road, Jinnan, Tianjin, 300222, China. gzgang1118@163.com.ORCID http://orcid.org/0000-0002-2978-7703

Funding

the Natural Science Foundation of Tianjin 21JCYBJC00560
6 · The paper itself

Abstract

Cardiomyocyte injury is a common complication during cardiac surgery with cardiopulmonary bypass (CPB). Studies have shown that circulating small extracellular vesicles (sEVs) are involved in the pathological process of cardiovascular diseases via delivering signaling molecules. This study aims to investigate the relationship between circulating sEV-encapsulated long noncoding RNAs (lncRNAs) and cardiac injury after CPB. Here, we found that the expression of sEV SEMA5A-IT1 in serum samples of patients after CPB was higher than that of pre-CPB serum samples. Moreover, serum-derived sEV SEMA5A-IT1 levels were negatively correlated with creatine kinase-MB (CK-MB) levels in patients who underwent CPB operation. Notably, circulating sEVs packaged with SEMA5A-IT1 could be uptaken by cardiomyocyte-like cells AC16 and increased SEMA5A-IT1 expression in AC16 cells. Upregulated SEMA5A-IT1 protected cardiomyocytes against hypoxia/reoxygenation injury, confirmed by increased cell viability, reduced cell apoptosis, and inhibited ferroptosis in AC16 cells. Mechanistically, SEMA5A-IT1 regulated the expression of B-cell CLL/lymphoma 2 (BCL2) and solute carrier family 7 member 11 (SLC7A11) through sponging miR-143-3p. Transfection of miR-143-3p mimics, BCL2, or SLC7A11 knockdown could attenuate the protective effect of SEMA5A-IT1 on cardiomyocytes. In conclusion, we propose that SEMA5A-IT1, which is transported to cardiomyocytes through circulating sEVs, is an important regulatory molecule that protects cardiomyocytes from ischemia-reperfusion injury, providing a target for the prevention and treatment of myocardial ischemia-reperfusion injury.

Indexed as

Cardiac Surgical ProceduresExtracellular VesiclesMicroRNAsMyocardial Reperfusion InjuryRNA, Long NoncodingSemaphorinsApoptosisCardiopulmonary BypassCreatine Kinase, MB FormHumansMyocytes, CardiacProto-Oncogene Proteins c-bcl-2Creatine Kinase, MB FormMicroRNAsProto-Oncogene Proteins c-bcl-2RNA, Long NoncodingSEMA5A protein, humanSemaphorinsApoptosisCardiomyocyte injuryCardiopulmonary bypassFerroptosismicroRNASmall extracellular vesicles

Identifiers

PMID36284269
PMCPMC9594885

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.