ArticleNature methods2022
ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- 3D multi-omics tumour atlases: from technology to biology and clinical translation.Nature reviews. Cancer · 2026Review
- Spatial omics illuminates tumor heterogeneity.Cell reports methods · 2026Review
- Spatial transcriptomics reveals influence of microenvironment on intrinsic fates in melanoma therapy resistance.Genome biology · 2026Article
- WDR5-H3K4me3 Epigenetic Axis Promotes TRMT6-Dependent tRNA MAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Visual Screening of Genetic Polymorphisms inSensors (Basel, Switzerland) · 2026Article
- Applications and development of in situ nucleic acid visualization techniques.Frontiers in bioengineering and biotechnology · 2026Review
- NuclampFISH enables cell sorting based on nuclear RNA expression for chromatin analysis.BMC genomics · 2025Article
- One probe fits all: a highly customizable modular RNA in situ hybridization platform expanding the application of SABER DNA probes.Development (Cambridge, England) · 2025Article
- Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER.Nature methods · 2025Article
- Homebuilt Imaging-Based Spatial Transcriptomics: Tertiary Lymphoid Structures as a Case Example.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Deconvolving organogenesis in space and time via spatial transcriptomics in thick tissues.bioRxiv : the preprint server for biology · 2024Article
- Generation of densely labeled oligonucleotides for the detection of small genomic elements.Cell reports methods · 2024Article
- Retrospective identification of cell-intrinsic factors that mark pluripotency potential in rare somatic cells.Cell systems · 2024Article
- Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Rapid in situ RNA imaging based on Cas12a thrusting strand displacement reaction.Nucleic acids research · 2023Article
- Enablers and challenges of spatial omics, a melting pot of technologies.Molecular systems biology · 2023Review
- Dual spatially resolved transcriptomics for human host-pathogen colocalization studies in FFPE tissue sections.Genome biology · 2023Article
- Technical Advances and Applications of Spatial Transcriptomics.GEN biotechnology · 2023Article
- Article
- Recent Advances in High-sensitivityActa histochemica et cytochemica · 2023Review
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
RNA labeling in situ has enormous potential to visualize transcripts and quantify their levels in single cells, but it remains challenging to produce high levels of signal while also enabling multiplexed detection of multiple RNA species simultaneously. Here, we describe clampFISH 2.0, a method that uses an inverted padlock design to efficiently detect many RNA species and exponentially amplify their signals at once, while also reducing the time and cost compared with the prior clampFISH method. We leverage the increased throughput afforded by multiplexed signal amplification and sequential detection to detect 10 different RNA species in more than 1 million cells. We also show that clampFISH 2.0 works in tissue sections. We expect that the advantages offered by clampFISH 2.0 will enable many applications in spatial transcriptomics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.