ArticleNature methods2022
Resolution doubling in light-sheet microscopy via oblique plane structured illumination.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 69 citations in OpenAlex.
- From stars to molecules: AI guided device-agnostic super-resolution imaging.Nature communications · 2026Article
- High-speed volumetric single-molecule imaging using dual-wavelength light sheets and PSF-engineered enhanced biplane detection.bioRxiv : the preprint server for biology · 2026Article
- High-Fidelity Long-term Whole-embryo Lineage and Fate Reconstruction by Iterative Tracking with Error Correction.bioRxiv : the preprint server for biology · 2026Article
- Article
- Real-time visualization of drug-target interactions in native subcellular microenvironments for lysosome-targeted drug discovery.Journal of pharmaceutical analysis · 2026Article
- When imaging technology meets single-cell omics: new paradigm in developmental biology.Cell regeneration (London, England) · 2025Review
- Altair-LSFM A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging.bioRxiv : the preprint server for biology · 2025Article
- Ultrack: pushing the limits of cell tracking across biological scales.Nature methods · 2025Article
- Super-resolution upgrade for deep tissue imaging featuring simple implementation.Nature communications · 2025Article
- Fast-adaptive super-resolution lattice light-sheet microscopy for rapid, long-term, near-isotropic subcellular imaging.Nature methods · 2025Article
- CELS-3D-Cutting edge light source for exciting fluorescence in microtome-based 3D microscopy and targeted correlative microscopy.Journal of anatomy · 2025Article
- Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM.Nature methods · 2025Article
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.Nature communications · 2024Article
- High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy.Nature communications · 2024Article
- Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging.Light, science & applications · 2024Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.bioRxiv : the preprint server for biology · 2024Article
- How Transcription Factor Clusters Shape the Transcriptional Landscape.Biomolecules · 2024Review
- Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.Nature communications · 2024Article
- Lightsheet microscopy integrates single-cell optical visco-elastography and fluorescence cytometry of 3D live tissues.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
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Authors and funding
16 authors at 6 institutions in 2 countries.
Funding
Abstract
Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence microscope without requiring high laser powers or specialized fluorophores. However, the excitation of out-of-focus fluorescence can accelerate photobleaching and phototoxicity. In contrast, light-sheet fluorescence microscopy (LSFM) largely avoids exciting out-of-focus fluorescence, thereby enabling volumetric imaging with low photobleaching and intrinsic optical sectioning. Combining SIM with LSFM would enable gentle three-dimensional (3D) imaging at doubled resolution. However, multiple orientations of the illumination pattern, which are needed for isotropic resolution doubling in SIM, are challenging to implement in a light-sheet format. Here we show that multidirectional structured illumination can be implemented in oblique plane microscopy, an LSFM technique that uses a single objective for excitation and detection, in a straightforward manner. We demonstrate isotropic lateral resolution below 150 nm, combined with lower phototoxicity compared to traditional SIM systems and volumetric acquisition speed exceeding 1 Hz.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.