ArticlePLoS genetics2022
Activation of the integrative and conjugative element Tn916 causes growth arrest and death of host bacteria.
Article in PLoS genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 15 citations in OpenAlex.
- Investigating the mobility and host range of mobile genetic elements harbouring antimicrobial resistance genes in enterococci.Microbiology (Reading, England) · 2026Article
- Bacterial evolution in the oral microbiome: the role of conjugative elements and horizontal gene transfer.Journal of bacteriology · 2025Review
- Activation and modulation of the host response to DNA damage by an integrative and conjugative element.Journal of bacteriology · 2025Article
- Identification of insertion sites for the integrative and conjugative element Tn916 in the Bacillus subtilis chromosome.PloS one · 2025Article
- Transcription termination and antitermination are critical for the fitness and function of the integrative and conjugative element Tn916.PLoS genetics · 2024Article
- Integrative and conjugative elements ofVirulence · 2024Article
- It's complicated: relationships between integrative and conjugative elements and their bacterial hosts.Current opinion in microbiology · 2024Review
- Genomic analysis of conjugative and chromosomally integrated mobile genetic elements in oral streptococci.Applied and environmental microbiology · 2024Article
- Activation and modulation of the host response to DNA damage by an integrative and conjugative element.bioRxiv : the preprint server for biology · 2024Article
- Microplastic-Mediated Transfer of Tetracycline Resistance: Unveiling the Role of Mussels in Marine Ecosystems.Antibiotics (Basel, Switzerland) · 2024Article
- Genomic Diversity of Methicillin-Resistant Staphylococcus aureus CC398 Isolates Collected from Diseased Swine in the German National Resistance Monitoring Program GEMicrobiology spectrum · 2023Article
- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.Microbial biotechnology · 2023Review
- TnSmu1 is a functional integrative and conjugative element in Streptococcus mutans that when expressed causes growth arrest of host bacteria.Molecular microbiology · 2022Article
- Activation of TnSmu1, an integrative and conjugative element, by an ImmR-like transcriptional regulator inMicrobiology (Reading, England) · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Integrative and conjugative elements (ICEs) serve as major drivers of bacterial evolution. These elements often confer some benefit to host cells, including antibiotic resistance, metabolic capabilities, or pathogenic determinants. ICEs can also have negative effects on host cells. Here, we investigated the effects of the ICE (conjugative transposon) Tn916 on host cells. Because Tn916 is active in a relatively small subpopulation of host cells, we developed a fluorescent reporter system for monitoring activation of Tn916 in single cells. Using this reporter, we found that cell division was arrested in cells of Bacillus subtilis and Enterococcus faecalis (a natural host for Tn916) that contained an activated (excised) Tn916. Furthermore, most of the cells with the activated Tn916 subsequently died. We also observed these phenotypes on the population level in B. subtilis utilizing a modified version of Tn916 that can be activated in the majority of cells. We identified two genes (orf17 and orf16) in Tn916 that were sufficient to cause growth defects in B. subtilis and identified a single gene, yqaR, that is in a defective phage (skin) in the B. subtilis chromosome that was required for this phenotype. These three genes were only partially responsible for the growth defect caused by Tn916, indicating that Tn916 possesses multiple mechanisms to affect growth and viability of host cells. These results highlight the complex relationships that conjugative elements have with their host cells and the interplay between mobile genetic elements.
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