Shared cis-regulatory modules control expression of the tandem paralogs midline and H15 in the follicular epithelium.

Cody A Stevens, Helen L Stott, Shreya V Desai, Nir Yakoby

Open access · greenAbstract read

In one paragraph

Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 51% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

Diversity ofFrontiers in cell and developmental biology · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Cody A StevensCenter for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.

Helen L StottCenter for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.

Shreya V DesaiDepartment of Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.

Nir YakobyCenter for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.ORCID 0000-0002-7959-7588

Rutgers, The State University of New Jersey · US

Funding

AREA: Mechanisms underlying epithelial cells’ patterning and morphogenesisR15GM101597 · NIGMS · RUTGERS THE STATE UNIV OF NJ CAMDEN · PI YAKOBY, NIR · 2013 to 2017

$806k

NIGMS NIH HHS R15 GM101597NIH HHS 2R15GM101597-02

6 · The paper itself

Abstract

The posterior end of the follicular epithelium is patterned by midline (MID) and its paralog H15, the Drosophila homologs of the mammalian Tbx20 transcription factor. We have previously identified two cis-regulatory modules (CRMs) that recapitulate the endogenous pattern of mid in the follicular epithelium. Here, using CRISPR/Cas9 genome editing, we demonstrate redundant activity of these mid CRMs. Although the deletion of either CRM alone generated marginal change in mid expression, the deletion of both CRMs reduced expression by 60%. Unexpectedly, the deletion of the 5' proximal CRM of mid eliminated H15 expression. Interestingly, expression of these paralogs in other tissues remained unaffected in the CRM deletion backgrounds. These results suggest that the paralogs are regulated by a shared CRM that coordinates gene expression during posterior fate determination. The consistent overlapping expression of mid and H15 in various tissues may indicate that the paralogs could also be under shared regulation by other CRMs in these tissues.

Indexed as

Drosophila ProteinsGene Expression Regulation, DevelopmentalAnimalsDrosophilaEpitheliumMammalsT-Box Domain ProteinsTranscription FactorsDrosophila ProteinsH15 protein, DrosophilaT-Box Domain ProteinsTranscription FactorsAnterior-posterior axis coordinationCis-regulationDevelopmental genesGene regulatory networkGenes taggingShared enhancers

Identifiers

PMID36278857

DOI10.1242/dev.201016

PMCPMC9845738

OpenAlexW4307179919

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.