Evidence map›Paper›PMID 36277728›Full record

ArticleFrontiers in endocrinology2022

cAMP-specific phosphodiesterase 8A and 8B isoforms are differentially expressed in human testis and Leydig cell tumor.

Federica Campolo, Chiara Capponi, Maria Grazia Tarsitano, Marta Tenuta, Carlotta Pozza, Daniele Gianfrilli, Fabio Magliocca, Mary A Venneri, Elena Vicini, Andrea Lenzi and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in endocrinology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. In the Brain of Phosphodiesterases: Potential Therapeutic Targets for Schizophrenia.Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology · 2025
    Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Federica CampoloDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Chiara CapponiDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy.
Maria Grazia TarsitanoDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Marta TenutaDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Carlotta PozzaDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Daniele GianfrilliDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Fabio MaglioccaDepartment of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Rome, Italy.
Mary A VenneriDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Elena ViciniDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy.
Andrea LenziDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Andrea M IsidoriDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Federica BarbagalloDepartment of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
Sapienza University of Rome · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cyclic adenosine monophosphate/Protein kinase A (cAMP/PKA) signaling pathway is the master regulator of endocrine tissue function. The level, compartmentalization and amplitude of cAMP response are finely regulated by phosphodiesterases (PDEs). PDE8 is responsible of cAMP hydrolysis and its expression has been characterized in all steroidogenic cell types in rodents including adrenal and Leydig cells in rodents however scarce data are currently available in humans. Here we demonstrate that human Leydig cells express both PDE8A and PDE8B isoforms. Interestingly, we found that the expression of PDE8B but not of PDE8A is increased in transformed Leydig cells (Leydig cell tumors-LCTs) compared to non-tumoral cells. Immunofluorescence analyses further reveals that PDE8A is also highly expressed in specific spermatogenic stages. While the protein is not detected in spermatogonia it accumulates nearby the forming acrosome, in the trans-Golgi apparatus of spermatocytes and spermatids and it follows the fate of this organelle in the later stages translocating to the caudal part of the cell. Taken together our findings suggest that 1) a specific pool(s) of cAMP is/are regulated by PDE8A during spermiogenesis pointing out a possible new role of this PDE8 isoform in key events governing the differentiation and maturation of human sperm and 2) PDE8B can be involved in Leydig cell transformation.

Indexed as

3',5'-Cyclic-AMP PhosphodiesterasesLeydig Cell TumorAdenosine MonophosphateHumansMaleProtein IsoformsSemen3',5'-Cyclic-AMP PhosphodiesterasesAdenosine MonophosphatePDE8A protein, humanPDE8B protein, humanProtein Isoformsacrosome biogenesishuman testisLeydig cellLeydig cell tumorPDE8APDE8B

Identifiers

PMID36277728
PMCPMC9585345
OpenAlexW4303616969

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.