Evidence map›Paper›PMID 36277416›Full record

ArticleVirusdisease2022

Molecular evidence of porcine circovirus 3 infection in swine: first report in southern India.

S Parthiban, A Ramesh, G Dhinakar Raj, Anbu Kumar Karuppannan, S Hemalatha, M Parthiban, Chintu Ravishankar, K Senthilkumar, D Balasubramaniyam

Abstract read
In one paragraph

Article in Virusdisease, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

S ParthibanDepartment of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College Campus, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007 India.ORCID 0000-0002-9852-4245
A RameshVaccine Research Centre-Viral Vaccines, CAHS, MMC, Chennai, 600 051 India.
G Dhinakar RajDepartment of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College Campus, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007 India.
Anbu Kumar KaruppannanVaccine Research Centre-Viral Vaccines, CAHS, MMC, Chennai, 600 051 India.
S HemalathaDepartment of Veterinary Pathology, MVC, Chennai, 600 007 India.
M ParthibanDepartment of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College Campus, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007 India.
Chintu RavishankarDepartment of Veterinary Microbiology, COVAS, Kerala Veterinary and Animal Sciences University, Pookode, India.
K SenthilkumarPost Graduate Research Institute in Animal Sciences, Kattupakkam, 603 203 India.
D BalasubramaniyamPost Graduate Research Institute in Animal Sciences, Kattupakkam, 603 203 India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study examined 434 field samples including serum (n = 273), swabs from natural orifices (n = 52) and postmortem tissue samples (n = 109) from both suspected and asymptomatic swine from Andhra Pradesh, Karnataka, Kerala, Pondicherry, Tamil Nadu, and Telangana states in southern India. All the samples were processed for molecular screening of PCV3 by specific PCR assay. Overall molecular positivity rate of PCV3 was found to be 0.7% in southern India with one sample positive from each state of Tamil Nadu, Kerala and Telangana. All the three PCR positive PCV3 samples are detected from reproductive failures and were processed and propagated in PK15 cell line for virus isolation. Out of 3 samples processed, one (INDKL9PK76) PCV3 isolate could be obtained in this study and it was confirmed by specific PCR at third and fifth passage levels. Sequencing of PCV3 positive PCR amplicon (INDKL9PK76) revealed 1004 nucleotides and BLAST analysis confirmed partial sequence of the PCV3 genome. The aligned contig sequence was submitted to GenBank under the accession number of MW627201. PCV3 sequence in this study revealed 99% homology with PCV3 isolates from Europe and China. Phylogentic analysis of the PCV3 isolate-INDKL9PK76 sequence along with established PCV3 genotypes revealed clustering within PCV3 genotypes. Characterization of PCV3 (INDKL9PK76) isolate based on deduced amino acid composition of PCV3-capsid protein revealed "A" (alanine) and "R" (arginine) at 24th and 27th residues respectively confirming the incidence of PCV3a genotype. This study evidences PCV3 associated reproductive failure in domestic pigs for the first time in southern India.

Indexed as

First reportMolecular detectionPCV3Southern IndiaSwine

Identifiers

PMID36277416
PMCPMC9481802

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.