ArticleVirusdisease2022
Colorimetric detection platform for banana bunchy top virus (BBTV) based on closed-tube loop mediated isothermal amplification (LAMP) assay.
Article in Virusdisease, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 citing papers in PubMed, 2 citations in OpenAlex.
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3 authors at 1 institution in 1 country.
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Abstract
A colorimetric closed-tube Loop mediated isothermal amplification (LAMP) assay was developed for rapid and sensitive detection of banana bunchy top virus (BBTV) from leaf and sucker tissues of infected banana plants. Six LAMP primers were designed targeting BBTV coat protein gene. Isothermal amplification was set at 65 °C and end point detection made by including hydroxy naphthol blue dye in the reaction where the positive samples showed colour change from violet to sky blue. Molecular characterization of LAMP amplicon was made with restriction digestion and sequencing. Restriction digestion with Supplementary Information: The online version contains supplementary material available at 10.1007/s13337-022-00784-w.
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