Evidence map›Paper›PMID 36274853›Full record

ArticleFrontiers in cell and developmental biology2022

A light sheet fluorescence microscopy protocol for

Jayson J Smith, Isabel W Kenny, Carsten Wolff, Rachel Cray, Abhishek Kumar, David R Sherwood, David Q Matus

Abstract read
In one paragraph

Article in Frontiers in cell and developmental biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Subcellular Imaging of Neuronal Calcium Handling In Vivo.Journal of visualized experiments : JoVE · 2023
    Article
  4. Tilt in Place Microscopy: a Simple, Low-Cost Solution to Image Neural Responses to Body Rotations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jayson J SmithDepartment of Neurobiology, University of Chicago, Chicago, IL, United States.
Isabel W KennyEmbryology: Modern Concepts and Techniques, Marine Biological Laboratory, Woods Hole, MA, United States.
Carsten WolffEmbryology: Modern Concepts and Techniques, Marine Biological Laboratory, Woods Hole, MA, United States.
Rachel CrayMarine Biological Laboratory, Woods Hole, MA, United States.
Abhishek KumarEmbryology: Modern Concepts and Techniques, Marine Biological Laboratory, Woods Hole, MA, United States.
David R SherwoodEmbryology: Modern Concepts and Techniques, Marine Biological Laboratory, Woods Hole, MA, United States.
David Q MatusEmbryology: Modern Concepts and Techniques, Marine Biological Laboratory, Woods Hole, MA, United States.

Funding

Understanding how cells invade through basement membrane in vivoR35GM118049 · NIGMS · DUKE UNIVERSITY · PI David R Sherwood · 2016 to 2026
$6.8M
Elucidating the mechanisms underlying cell cycle regulation of invasive behaviorR01GM121597 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI MATUS, DAVID · 2017 to 2021
$2.1M
A comprehensive toolkit to visualize endogenously tagged fluorescent basement membrane components in a living animalR21OD028766 · OD · DUKE UNIVERSITY · PI SHERWOOD, DAVID R · 2020 to 2021
$427k
A Comprehensive Endogenous Basement Membrane Toolkit to Elucidate how Basement Membranes Stretch on Mechanically Active Tissues and Decline during AgingR21OD032430 · OD · DUKE UNIVERSITY · PI SHERWOOD, DAVID R · 2022 to 2023
$427k
NIGMS NIH HHS R01 GM121597NIGMS NIH HHS R35 GM118049NIH HHS R21 OD028766NIH HHS R21 OD032430
6 · The paper itself

Abstract

Light sheet fluorescence microscopy (LSFM) has become a method of choice for live imaging because of its fast acquisition and reduced photobleaching and phototoxicity. Despite the strengths and growing availability of LSFM systems, no generalized LSFM mounting protocol has been adapted for live imaging of post-embryonic stages of

Indexed as

BIO-133C. eleganslight sheet fluorescence microscopypostembryonic developmenttimelapse

Identifiers

PMID36274853
PMCPMC9586288

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.