ArticleMolecular biology reports2022
Transcript expression level analysis of phytoene synthase and phytoene desaturase associated with β-carotene content in selected Kenyan Bitter melon.
Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 4 citations in OpenAlex.
- Molecular insights into charantin and β-carotene biosynthesis in bitter gourd (Momordica charantia L.): Gene expression dynamics and metabolite profiling.Journal, genetic engineering & biotechnology · 2025Article
- Influence of TriploidPlants (Basel, Switzerland) · 2025Article
- Plant regeneration from embryogenic callus-derived from immature leaves of Momordica charantia L.Heliyon · 2023Article
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBitter melon (Momordica charantia L.) is a widely cultivated food and medicinal plant native to the world's subtropics and tropics. Constraints affecting cultivation of Bitter melon affect productivity of β-carotene. Knowing the mechanism that controls the transcription of the β-carotene biosynthesis genes in Bitter melon will be of great value in improving the yield of this important metabolite. METHODS AND
resultsThe expressions of β-carotene biosynthetic genes such as Phytoene Desaturase (PDS) and Phytoene Synthase (PSY) were evaluated in Bitter melon accessions 'GBK027049', 'NS1026', 'Mahy-ventura', '453B' and 'Sibuka532'. Transcript expression level analysis of PSY and PDS, and amount of β-carotene in leaf, stem, and fruit, were determined using quantitative polymerase chain reaction and high-performance liquid chromatography (HPLC). Root transcript expression was used as a negative control for determining relative fold change in other tissues. Expression of PSY in fruit (6 to 27-fold compared to the control) was higher than in the other organs for all accessions. This was also the case of PDS expression (10 to 29-fold compared to the control). Leaves had the highest β-carotene concentration (17.92-45.35 µg∙g
conclusionBitter melon leaves, especially those of 'GBK027049' and '453B' accessions, are an alternative to alleviate the β-carotene deficiencies in the world and especially in Africa.
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